SU Jia,GAO Zeng-gui,YAO Yuan,et al.Assessment of Genetic Diversity Among Fusarium Wilt Pathogens of the Cucurbits Detected by ISSR Markers[J].Northern Horticulture,2014,38(17):97-101.
利用ISSRPCR分子标记技术分析瓜类枯萎病病原菌遗传多样性
- Title:
- Assessment of Genetic Diversity Among Fusarium Wilt Pathogens of the Cucurbits Detected by ISSR Markers
- 文章编号:
- 1001-0009(2014)17-0097-05
- Keywords:
- Fusarium oxysporum; ISSR-PCR; genetic diversity
- 分类号:
- S436.42
- 文献标志码:
- A
- 摘要:
- 利用ISSR分子标记技术对32株不同的尖孢镰孢菌进行遗传多样性分析，从遗传多样性角度探讨尖孢镰孢菌专化型。结果表明：11条引物共扩增出109条带，其中多态性条带99条，占总条带数的90.82%,扩增的条带大小为120～2 000 bp。供试的32株尖孢镰孢菌的遗传相似系数在0.64～0.91，在相似系数0.705的条件下菌株被分为5个类群。同一类群中，相同寄主菌株之间的遗传相似性与菌株的地理来源无明显的相关性。
- Abstract:
- Genetic variation among 32 Fusarium oxysporum isolates was analysed by using inter-simple sequence repeat (ISSR) polymorphism,forma specialis of Fusarium oxysporum Schl.were researched from the perspective of genetic diversity.The results showed that a total of 109 bands were amplified with 11 ISSR primers,99 bands were polymorphic,the rate was 90.82%.The amplified fragment sizes ranged from 120 to 2 000 bp.The genetic similarity among 32 F.oxysporum isolates ranged from 0.64 to 0.91 and all the strains could be divided into 5 groups at the level of 0.705.In the same group,there was no distinct relation between the genetic similarity and geographic origin of the strains isolated from the same hosts.
参考文献/References:
［1］Booth C.镰刀菌属［M］.陈其煐，译.北京:中国农业出版社,1988.
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备注/Memo
第一作者简介：苏家（1987-），女，黑龙江齐齐哈尔人，硕士研究生，研究方向为蔬菜病害。E-mail：baobaoxiang@126.com.