XU Fang-fang,ZHAO Li-li,XUN Yang,et al.Optimization of Media for Inducing Ovule Culture of ?Platycodon grandiflorum[J].Northern Horticulture,2014,38(15):106-108.
桔梗胚珠培养中愈伤组织诱导培养基的优化
- Title:
- Optimization of Media for Inducing Ovule Culture of ?Platycodon grandiflorum
- 文章编号:
- 1001-0009(2014)15-0106-03
- 分类号:
- Q949.783.2
- 文献标志码:
- A
- 摘要:
- 以桔梗未受精胚珠为试材,研究比较了不同生长素和细胞分裂素组合对其愈伤组织诱导及鲜重大小的影响,以筛选最佳诱导培养基。结果表明:诱导桔梗未受精胚珠愈伤组织形成的比较理想的培养基组合有2个,一是N6+1.0 mg/L 2,4-D+0.1 mg/L KT,其诱导率最高,达到了100%,同时愈伤组织平均鲜重较大;二是N6+0.5 mg/L NAA+0.1 mg/L KT,其诱导率较高为90%,愈伤组织平均鲜重最大。
- Abstract:
- Taking unfertilized ovules of Platycodon grandiflorum as materials,the effect of different combinations of auxin and cytokinin on the callus induction and fresh weight were studied and compared,in order to screen out the best induction medium.The results showed that two optimal media for inducing unfertilized ovules to form calli were selected,the first optimal medium was N6+1.0 mg/L 2,4-D+0.1 mg/L KT,the induction rate was 100% and the average fresh weight of callus was large; the second optimal medium was N6+0.5 mg/L NAA+0.1 mg/L KT,the induction rate was 90% and the average fresh weight of callus was the largest.
参考文献/References:
[1]朱社敏,费远志.各种桔梗溶血作用的比较[J].中草药,1991,22(7):324-325.
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备注/Memo
第一作者简介:徐芳芳(1989-),女,吉林白城人,硕士研究生,现主要从事中药材桔梗的组织培养等研究工作。E-mail:xff615@163.com.