[1]程华,李琳玲,袁红慧,等.内源激素含量变化与板栗花芽分化关系研究[J].北方园艺,2013,37(22):5-9.
　CHENG Hua,LI Lin-ling,YUAN Hong-hui,et al.Study on the Relationship of Changes of the Endogenous Hormone Content in Castanea mollissima and Bud Differentiation[J].Northern Horticulture,2013,37(22):5-9.

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内源激素含量变化与板栗花芽分化关系研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷期数: 2013年22期页码: 5-9 栏目: 出版日期: 2013-11-30

Title:: Study on the Relationship of Changes of the Endogenous Hormone Content in Castanea mollissima and Bud Differentiation

文章编号:: 1001-0009(2013)22-0005-05

作者:: 程华¹; 2; 李琳玲¹; 2; 袁红慧¹; 2; 徐向阳¹; 王燕¹; 3; 程水源¹; 2; 1.经济林木种质改良与资源综合利用湖北省重点实验室，湖北黄冈 438000；
2.黄冈师范学院化学与生命科学学院，湖北黄冈 438000；
3.湖北省林业厅科技教育处，湖北武汉 430079

Author(s):: CHENG Hua¹; 2; LI Lin-ling¹; 2; YUAN Hong-hui¹; 2; XU Xiang-yang¹; WANG Yan¹; 3; CHENG Shui-yuan¹; 2; 1.Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories,Huanggang,Hubei 438000;
2.College of Chemistry and Life Science,Huanggang Normal University,Huanggang,Hubei 438000;
3.Technology Education Department,Forestry Department of Hubei Province,Wuhan,Hubei 430079

关键词:: 板栗; 内源激素; 比值; 变化

Keywords:: Castanea mollissima; endogenous hormones; ratio; variation

分类号:: S 664.2

文献标志码:: A

摘要:: 以罗田板栗主栽品种“八月红”、“乌壳栗”、“中果早栗”、“玫瑰红”为试材，采用高效液相色谱方法，研究了板栗花芽分化不同时期体内吲哚乙酸（IAA）、赤霉素（GA₃）、玉米素核苷（ZR）、脱落酸（ABA）等激素含量的变化。结果表明：在板栗冬前梢花芽分化过程中，IAA含量一直呈上升态势，并保持在一个较高水平；而ABA含量则呈先上升后下降、又略有上升趋势；GA₃含量呈先上升后下降、之后保持在一个相对较低含量水平状态；ZR含量呈先上升后缓慢下降趋势。综合分析表明，IAA对板栗冬前梢花芽分化影响较大；低含量的ABA有利于板栗花簇原基的分化和生长，对花芽分化进程的影响有待进一步研究；GA3有利于花芽打破休眠其向花序原基分化的转化，且花序原基分化期与花簇原基分化期均需维持一定含量的GA₃；高含量的ZR可能对板栗花芽分化具有促进作用。在对ZR/IAA、GA₃/IAA、ABA/IAA、GA₃/ZR值分析过程中发现,ZR/IAA、GA₃/IAA、ABA/IAA值均呈现出先上升后下降的变化趋势，而GA₃/ZR值则一直下降，之后保持在一个较低的水平。从整个板栗花芽分化过程中内源激素比例分析看，较高水平ZR/IAA、GA₃/IAA及ABA/IAA值有利于板栗冬前梢打破休眠期进入花芽分化时期，但由花序原基进入花簇原基分化却需要较低水平的ZR/IAA、GA₃/IAA及ABA/IAA值。通过内源激素比例变化趋势分析说明，板栗冬前梢花芽分化的启动和各阶段的进程与各内源激素的平衡有密切联系。

Abstract:: In order to evaluate the relationship between floral bud differentiation and the hormone changes (IAA,GA₃,ZR,ABA) in chestnut,the major cultivars,‘Bayuehong’,‘Wukeli’,‘Zhongguozaoli’,‘Meiguihong’ were selected as the testing materials.The relationship was analyzed by HPLC.The results showed that the IAA content had showed a rising state during the process of flower bud differentiation in chestnut and maintained at a higher revel.ABA content increased and then decreased and rised at last.GA₃ content increased at first and then decreased.It maintained at a low content state at last.ZR content increased firstly and then slowed down in the process.Comprehensive analysis showed chestnut bud tip before winter required a certain concentration of IAA,IAA had larger impact on bud stage;the low level of ABA was beneficial to differentiation and growth of chestnut flower;GA₃ was helpful to break dormancy and it was favorable to conversing to the inflorescence primordium and inflorescence primordium.Primordium and the flower clusters required to maintain certain levels of GA₃,and high levels of ZR may had a promoting effect on the chestnut bud.In the analysis process of ZR/IAA,GA₃/IAA,ABA/IAA and GA₃/ZR value,ZR/IAA,GA₃/IAA and ABA/IAA showed a step change.It was a process of first increase and decrease followly,while GA₃/ZR value had been declining,and then maintained a lower value.The proportion of endogenous hormone in the chestnut bud in the entire process was analysed,high levels of ZR/IAA,GA₃/IAA and ABA/IAA were conducive to chestnut shoots before winter breaking into flower bud dormancy period,but the period of the inflorescence rimordial into flower clusters rimordial required a lower level of ZR/IAA,GA₃/IAA and ABA/IAA.Proportion of endogenous hormones through trend analysis showed that chestnut flower bud tip before winter and the various stages of the process with the endogenous hormone balance were closely linked.

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备注/Memo

第一作者简介：程华(1980-)，男，湖北麻城人，博士，讲师，现主要从事经济林种质资源评价与利用等研究工作。E-mail:chenghua1437@126.com.
责任作者：程水源(1965-)，男，博士，教授，博士生导师，现主要从事经济林种质资源评价与利用等研究工作。E-mail：s_y_cheng@sina.com.
基金项目：湖北省自然科学基金重点资助项目(2010CBB03901)；
湖北省教育厅高校产学研合作重点资助项目(C2010060)；
2011中央财政林业科技推广示范资助项目(2011BH0032)。
收稿日期：2013-09-23

更新日期/Last Update: 2014-08-06