NI Xin-jiang,CHU Yang,ZHAO Mei,et al.Activity Changes of Several Extracellular Enzymes from Three Pholiota adipose Strains at Different Stages of Development[J].Northern Horticulture,2013,37(21):153-156.
三个黄伞菌株不同生长发育期几种胞外酶活性变化
- Title:
- Activity Changes of Several Extracellular Enzymes from Three Pholiota adipose Strains at Different Stages of Development
- 文章编号:
- 1001-0009(2013)21-0153-04
- 分类号:
- S 646.1+9
- 文献标志码:
- A
- 摘要:
- 以3个黄伞菌株为试材，以棉籽壳为主料，采用瓶栽方法对其不同生长发育期的几种胞外酶活性进行了测定，以期揭示同种食用真菌不同菌株间的相同胞外酶活性变化特点。结果表明：3个黄伞菌株均具有完整的胞外纤维素酶系；3个黄伞菌株的胞外羧甲基纤维素酶（CMC酶）、滤纸纤维素酶（FP酶）、半纤维素酶（CH酶）、淀粉酶和果胶酶活性高峰均对应于子实体生长发育期；在整个栽培期，3个黄伞菌株的胞外β-葡萄糖苷酶均无活性高峰出现；3个黄伞菌株的漆酶在菌丝满瓶期和潮菇间期均有活性高峰出现，在头潮菇和2潮菇子实体成熟期，漆酶活性均处于较低水平；3个黄伞菌株的胞外过氧化物酶在菌丝生长期均具有较高活性，头潮菇原基期后，过氧化物酶活性均显著下降。可见3个黄伞菌株的相同胞外酶的活性变化趋势大致相同。
- Abstract:
- Taking three Pholiota adipose strain as materials,the activity changes of several extracellular enzymes were determined at different stages of development from three Pholiota adipose strains cultivated on cotton seed hull in bottle to reveal the features of activity changes of the same extracellular enzymes from different strains of the same edible fungi.The results indicated that all of the three strains had complete set of extracellular cellulose；the activity peaks of extracellular carboxymenthel cellulase (CMCase)，filter paper cellulase (FPase)，hemicellulase (HCase)，amylase and pectase from the three strains all appeared in the period between primordial formation and fruit-body maturity；there were no great changes in β-glucosidase activity from the three strains throughout its growth；the activity peaks of extracellular laccase from the three strains appeared in the period when substrates were fully colonized and in the period between first and second flush but their activities were low in the fruit-body maturity of both the first and second flush；and the extracellular peroxidase activities from the three strains remained high in the period of mycelial growth and then declined rapidly at the beginning of fruiting.It could be concluded that the activity changes of the same extracellular enzymes from the three strains were roughly the same.
参考文献/References:
[1]王立安，陈惠.滑菇与黄伞[M]．北京：中国农业出版社，2009. [2]田景花，杨旭，李明，等.黄伞菌种培养基配方筛选研究[J]．北方园艺，2013(2)：137-140. [3]刘靖宇，梁志英，常明昌，等．珍稀食药用菌黄伞生产工艺研究[J]．食用菌学报，2006，13(2)：81-84. [4]姜华，黄清荣，臧丽丽，等．黄伞菌丝蛋白质营养价值评价[J]．菌物学报，2005，24(3)：441-445. [5]刘靖宇，孟俊龙，张江萍，等.三种培养料对黄伞胞外酶活性的影响[J].食用菌学报，2006，13(1)：41-43. [6]Mandels M，Hontz L，Nystrom J，et al.Enzymatic hydrolysis of waste cellulase[J].Biotechnol Bioeng，1974（16）：1471-1493. [7]王玉万，王云.构菌栽培过程中对木质纤维素的降解和几种多糖分解酶的活性变化[J].微生物学通报，1989，16(3)：137-140. [8]Sengupta S，Sengupta S.β-Glucosidase production by the mycelial culture of the mushroom?Termitomyces clypeatus[J].Enzyme Microb Technol,1990（12）：309-314. [9]Shamala T R，Sreekantiah K R．Production of cellulases and D-xylanase by some selected fungal isolases[J].Enzyme Microb Technol，1986,8(3)：178-182. [10]潘迎捷，陈明杰，郑海歌，等．香菇和平菇生长发育中漆酶、酪氨酸酶和纤维素酶活性的变化[J]．上海农业学报，1991，7(2)：21-26. [11]张志良，瞿伟青．植物生理学实验指导[M]．2版.北京：高等教育出版社，1990. [12]初洋，倪新江，杨桂文，等．姬菇和鲍鱼菇生长期间8种胞外酶活性变化比较[J]．烟台大学学报(自然科学与工程版)，2008，21(2)：138-142. [13]倪新江，丁立孝，潘迎捷，等．姬松茸在两种培养基上生长期间九种胞外酶活性变化[J]．菌物系统，2001，20(2)：222-227. [14]刘朝贵，高金权，李成琼．糙皮侧耳(Pleurotus ostreatus)降解转化稻草秸秆研究[J]．西南农业大学学报(自然科学版)，2006，28(2)：258-263. [15]路等学，王龙，高静梅，等．茶薪菇不同生长期几种胞外酶活性的测定[J]．华北农学报，2009，24(z1)：275-277. [16]王玉江，韩增华，戴肖东，等．黑木耳栽培过程中胞外酶活性变化[J]．食用菌学报，2010，17(4)：40-43.
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备注/Memo
第一作者简介：倪新江（1955-），男，硕士，教授，现主要从事食用菌营养生理等研究工作。E-mail：nxjzhen@sina.com.