[1]刘幸佳,徐伟韦,崔玉花,等.春兰组培快繁技术研究[J].北方园艺,2013,37(20):101-104.
　LIU Xing-jia,XU Wei-wei,CUI Yu-hua,et al.Study on Tissue Culture and Rapid Propagation of Cymbidium goeringii[J].Northern Horticulture,2013,37(20):101-104.
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春兰组培快繁技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年20期 页码: 101-104 栏目: 出版日期: 2013-10-30
Title:
Study on Tissue Culture and Rapid Propagation of Cymbidium goeringii
文章编号:
1001-0009(2013)20-0101-04
作者:
刘幸佳1; 徐伟韦2; 崔玉花3; 崔永一4
1.浙江农林大学 风景园林与建筑学院，浙江 临安 311300；
2.慈溪市农业技术推广中心，浙江 慈溪 315300；
3.吉林省龙井市农村能源环保办公室，吉林 龙井 133400；
4.浙江农林大学 农业与食品科学学院，浙江 临安 311300
Author(s):
LIU Xing-jia1; XU Wei-wei2; CUI Yu-hua3; CUI Yong-yi4
1.School of Landscape Architecture，Zhejiang Agricultural and Forestry University，Lin’an，Zhejiang 311300；
2.Cixi Agriculture Technology and Popularization Center，Cixi，Zhejiang 315300；
3.Office of Rural Energy and Environmental Protection in Longjing of Jilin Province，Longjing，Jinlin 133400；
4.School of Agricultural and Food Science，Zhejiang Agricultural and Forestry University，Lin’an，Zhejiang 311300
关键词:
春兰; 根状茎; 组培; 快繁
Keywords:
Cymbidium goeringii; rhizomes; tissue culture; rapid propagation
分类号:
S 682.31
文献标志码:
A
摘要:
以春兰‘宋梅’根状茎为试材，研究了NAA及活性炭对春兰根状茎增殖的影响，并通过正交设计筛选有利于根状茎芽分化和幼苗生根的培养基。结果表明：NAA和活性炭均能不同程度促进根状茎的增殖与生长；1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L为根状茎芽分化的最适培养基；1/2MS+IBA 1.0 mg/L+活性炭2.0 g/L为春兰组培苗生根的最适培养基。
Abstract:
With rhizomes of Cymbidium goeringii ‘Songmei’ as explants，the effects of plant growth regulators (NAA) and activated charcoal on multiplication of rhizomes were studied，the media components for differentiation and rooting were screened by orthogonal test.The results showed that NAA and activated charcoal could promote the multiplication and growth of rhizomes in different degrees.For differentiation it was the best on 1/2MS medium containing 1.0 mg/L 6-BA and 0.1 mg/L NAA.As for rooting，1/2MS supplemented with 1.0 mg/L IBA and 2.0 g/L activated charcoal was the best.

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备注/Memo

第一作者简介：刘幸佳（1988-），女，硕士研究生，现主要从事兰科植物的组织培养及其菌根真菌研究等工作。E-mail:liuxingjia880228@126.com.

责任作者：崔永一（1966-），男，博士，教授，硕士生导师，现主要从事兰科植物品种改良和生理生化及分子生物学研究等工作。E-mail:orchidcui@163.com.
基金项目：浙江省温州科技局国际合作资助项目（H20080049）；浙江省新苗人才计划资助项目（2008R40G2100075）；浙江省农业科学院国际合作专项经费资助项目（2045200517）。

更新日期/Last Update: 2014-08-26