SHI Bao-ping,LI Cheng-liang,DU Ye-juan,et al.Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang[J].Northern Horticulture,2013,37(19):114-117.
新疆田旋花丛簇病植原体的分子鉴定
- Title:
- Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang
- 文章编号:
- 1001-0009(2013)19-0114-04
- 分类号:
- Q 813.1
- 文献标志码:
- A
- 摘要:
- 为明确新疆田旋花丛簇病植原体的分类地位，现利用植原体16S rRNA基因通用引物P1/P7和R16F2n/R16R2对新疆石河子地区表现丛簇症状的田旋花植株总DNA进行巢式PCR扩增，并对扩增片段进行克隆和测序。结果表明：田旋花丛簇病植原体16S rRNA基因片段长1 229 bp（Genbank No.：KC414725），该分离物在系统发育树中与植原体16SrII组花生丛枝组（Peanut witches’broom）成员聚集在一起，与该组成员中的茄子巨芽Eggplant big bud（JX083377）植原体同源性最高，达到98％。因此确定田旋花丛簇病植原体是16SrII组成员。
- Abstract:
- In order to identify the taxonomic status of phytoplasma of Convolvulus arvensis cluster disease in Xinjiang，using phytoplasma universal primers P1/P7 and R16F2n/R16R2 for 16S rRNA gene to detect phytoplasma in Convolvulus arvensis showing symptoms of cluster disease in Xinjiang Shihezi area.The target fragment was amplified from the total DNA by nested-PCR，then it was cloned and sequenced.The results showed that the 16S rRNA gene of Convolvulus arvensis cluster phytoplasma consists of 1 229 bp (Genbank No.:KC414725)，it clustered together with the members of phytoplasma Peanut witches’broom (16SrII) group，and shared a high similarity(98％)with the phytoplasma EBB-A (Eggplant big bud） belongs to 16SrII.So it was confirmed that the phytoplasma strain Convulus arvensis cluster disease was the member of phytoplasma 16SrII group.
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备注/Memo
第一作者简介：石宝萍（1985-），女，硕士研究生，研究方向为植物病毒与病害防治。