HAN Rui,WANG Xian-lei,LI Guan.Cloning and Analysis of Partial Sequence of Resistance to Powdery Mildew Candidate Gene from Cucumis melon L.[J].Northern Horticulture,2013,37(19):110-113.
甜瓜抗白粉病候选基因片段克隆与序列分析
- Title:
- Cloning and Analysis of Partial Sequence of Resistance to Powdery Mildew Candidate Gene from Cucumis melon L.
- 文章编号:
- 1001-0009(2013)19-0110-04
- Keywords:
- melon; powdery mildew candidate gene; gene cloning; sequence analysis
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以2个高感白粉病的甜瓜品种“K413”、“JS”和3个高抗品种“H70”、“AN”、“H74”为试材，采用PCR方法,对第Ⅴ连锁群的Pm-AN片段进行克隆分析。结果表明：从“K413”、“JS”、“H70”、“AN”和“H74”中克隆出Pm-AN-N/C 2个片段分别得到830 bp和610 bp 2个碱基，分别编码氨基酸286个和192个，同源性分别为99.74%和94.00%。cDNA扩增结果表明，5个甜瓜品种分别出现5种不同的带型，在“JS”、“H70”、“AN”中还出现了2种不同的带型，而且所有带型都有一定的差异。
- Abstract:
- Taking two high sensitive melon powdery mildew varieties ‘K413’，‘JS’ and three high resistant varieties ‘H70’，‘AN’，‘H74’ as test materials，using PCR method，part V linkage group Pm-AN was cloned and analyzed.The results showed that the cloned Pm-AN-N/C two pieces were 830 bp and 610 bp respectively from ‘K413’，‘JS’，‘H70’，‘AN’ and ‘H74’，encoding amino acids 286 and 192 respectively，homology of 99.74% and 94.00% respectively.cDNA amplification results showed that the five melon varieties appeared five different band type，respectively，appearing in two different type from ‘JS’，‘H70’，‘AN’，and all model had difference.
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备注/Memo
第一作者简介：韩瑞（1987-），女，硕士研究生，现主要从事植物分子生物学研究工作。E-mail:hanrui1009@sina.com.