JIANG Yong-lei,LU Hong-ding,HUANG Xiao-xia,et al.Effect of Plumbum Stress on Physiological Characteristics of Machilus yunnanensis Seedlings[J].Northern Horticulture,2013,37(19):75-79.
铅胁迫对滇润楠幼苗生理特性的影响
- Title:
- Effect of Plumbum Stress on Physiological Characteristics of Machilus yunnanensis Seedlings
- 文章编号:
- 1001-0009（2013）19-0075-05
- Keywords:
- plumbum（Pb） stress; Machilus yunnanensis; chlorophyll fluorescence; physiological characteristics
- 分类号:
- S 687.1
- 文献标志码:
- A
- 摘要:
- 以2 a生滇润楠实生幼苗为试材，研究了对照（CK）、5 mmol/L Pb(NO3)2、10 mmol/L Pb(NO3)2铅胁迫处理对其部分生理生化特性的影响。结果表明：Pb2+胁迫处理下，叶片初始荧光(F0)呈现上升趋势，PSII最大光化学效率（Fv/Fm）和PSII实际光合效率（Yield）显著受Pb2+的影响，且在低浓度的Pb2+浓度胁迫下呈现出显著下降趋势，表明在Pb(NO3)2溶液处理下的滇润楠幼苗光系统II受到损伤，且5 mmol/L浓度处理比10 mmol/L浓度处理对其伤害更大。此外，Pb2+胁迫引起植株叶绿素含量先降后升、可溶性蛋白含量显著下降，导致膜脂过氧化产物丙二醛（MDA）含量的增加，且在5 mmol/L的处理浓度下的变化较大。同时Pb2+胁迫使过氧化物酶（POD）活性显著提高，在低浓度铅胁迫下脯胺酸含量显著降低，表明该幼苗可以通过启动抗氧化酶系统及渗透调节来抵御铅胁迫。
- Abstract:
- Taking two-year old seedlings of Machilus yunnanensis as experiment material，the effect of some physiological and biochemical characteristics under three stress treatment groups control (CK)，5 mmol/L Pb(NO3)2，10 mmol/L Pb(NO3)2 were studied.The results showed that under the Pb2+ treatment，the initial fluorescence (F0) increased，while the maximal photochemical efficiency of PSII (Fv/Fm) and the actual photochemical efficiency of PSII (Yield) decreased significantly in the low Pb2+concentration，which indicating the PSII of Machilus yunnanensis seedlings was damaged by Pb(NO3)2treatment，especially by the 5 mmol/L concentration of Pb(NO3)2 treatment.Furthermore，the contents of chlorophyll dropped and then increased as Pb2+ concentration raised continually.Soluble protein contents declined obviously，and significantly stimulated the increase of MDA content；moreover，these changes were more evident under the 10 mmol/L concentration treatment.On the other hand，the Pb2+stress promoted the POD activity and decreased the accumulation of praline significantly in low concentration of Pb2+，which suggested the Machilus yunnanensis seedlings could resist Pb2+stress by its antioxidative enzyme and osmotic regulation system.
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备注/Memo
第一作者简介：姜永雷（1988-），男，河南周口人，硕士研究生，研究方向为园林植物。E-mail：10-29jyl@163.com.