GAO Xiang-yang,WANG Ying-ying,WANG Shan.Determination of Forchlorfenuron in Garden Stuff by Flow Injection chemiluminescence with Dispersive Solid-Phase Extraction[J].Northern Horticulture,2013,37(19):26-29.
分散固相萃取-流动注射化学发光法速测果蔬中残留氯吡脲研究
- Title:
- Determination of Forchlorfenuron in Garden Stuff by Flow Injection chemiluminescence with Dispersive Solid-Phase Extraction
- 文章编号:
- 1001-0009(2013)19-0026-04
- Keywords:
- dispersive solid-phase extraction; flow injection-chemiluminescence; garden stuff; forchlorfenuron
- 分类号:
- O 657.39
- 文献标志码:
- A
- 摘要:
- 以西瓜、草莓、葡萄、猕猴桃、西红柿、黄瓜等为测试样品，样品中的氯吡脲经乙腈提取、N-丙基乙二胺（PSA）分散固相萃取（DSPE）净化，利用其对luminol-KIO4化学发光体系的增敏作用进行了测定，以期建立的分散固相萃取-流动注射化学发光法快速测定果蔬中残留氯吡脲的新方法。结果表明：该方法的线性范围为1.0×10-11～1.0×10-5 mol/L，相关系数r=0.9993，对1.0×10-10 mol/L氯吡脲11次平行测定的相对标准偏差为1.5%，按3倍标准偏差计算的方法检出限为0.163 ng/g，加标回收率为89.7%～98.9%。表明该法线性范围宽、灵敏度高、设备简单，其用于测定果蔬中残留氯吡脲的结果比较理想。
- Abstract:
- Taking watermelon，strawberry，grape，kiwifruit，tomato，cucumber as materials，a new method was established for determination of forchlorfenuron in garden stuff by flow injection-chemiluminescence with dispersive solid-phase extraction.Forchlorfenuron was extracted with acetonitrile and purified with primary secondary amine.Since forchlorfenuron could enhanced the reaction between luminol and potassium periodate，it could be detected by flow injection chemiluminescence method.The results showed that the linear ranges was 1.0×10-11～1.0×10-5 mol/L，the correlation coefficient was 0.9993，the detection limit was 0.163 ng/g，the relative standard deviation (RSD)was 1.5% for 11 parallel determination，and the recovery between 89.7%～98.9%.This method has been successfully applied to the determination of forchlorfenuron in garden stuff samples because of the wide linear ranges and high sensitive.
参考文献/References:
[1]齐明芳.三种常用坐果激素主要成分在番茄中的残留[J].农药,2010,49(7):512-513. [2]齐明芳.氯吡脲在甜瓜果实中的残留分析[J].江苏农业科学,2010,39(6):486-487. [3]聂磊,刘鸿先.植物生长调节剂影响沙田柚内源激素水平的研究[J].生态科学,2001,20(3):44-50. [4]边庆花,文辉,侯士聪,等.1-(2-氯-4-吡啶基）-3-苯基脲的高效液相色谱分析[J].化学试剂,2005,27(6):361-362. [5]张锋锋,苟金萍,姜瑞.氯吡脲在设施甜瓜中的消解动态与残留测定[J].中国农学通报,2011,27(25):200-204. [6]孙大利,郑尊涛,叶火春,等.氯吡脲在葡萄中的残留分析方法及消解动态[J].农药,2011,50(10):751-753. [7]高立明,王广成,吴春先,等.氯吡脲的高效液相分析方法[J].农药科学与管理,2007,25(2):9-12. [8]Liu Q,Gong D X,Peng X C，et a1.Determination of forchlorfenuron dissipation and residue in cucumbers and red soil[J].Journal of Northeast Agricultural University,2010,17(1):27-31. [9]陈长龙,李建中,王会利,等.氯吡脲在土壤和西瓜中的残留分析[J].环境化学,2006,25(6):789-792. [10]徐永,寿林飞,虞淼,等.超高效液相色谱-串联四级杆质谱联用法测定水果中多效唑、氯吡脲和咪鲜胺的残留[J].农药学学报,2012,14(1):61-66. [11]姜欣,周宁.调吡脲的气相色谱分析[J].农药,1996,35(9):21-22. [12]Suarez-Pantaleon C,Mercader J V,Agullo C,et a1.Production and characterization of monoclonal and polyclonal antibodies to forchlorfenuron[J].Journal of Agricultural and Food Chemistry,2008,56(23):11122-11131. [13]张莹,鹿毅,杨涛,等.高效液相色谱-串联质谱法测定果蔬中7种植物生长促进剂残留[J].分析测试学报,2012,31(4):442-447. [14]郝开拓,孔祥虹,杜宝中,等.分散固相萃取-气相色谱-质谱法分析蕨菜、黑米中53种农药残留量[J],分析实验室,2011,30(6):69-74. [15]刘浩,周芳,李月娥.分散固相萃取法对果蔬中农药残留前处理的优化[J].环境科学与管理,2008,33(5):137-140. [16]胡江涛,盛毅,方智,等.分散固相萃取-高效液相色谱法快速检测猕猴桃中的氯吡脲[J].色谱,2007,25(3):441-442. [17]毛建霏，雷绍荣,郭灵安,等.QuEChERs 前处理快速测定豆芽中氯吡脲残留[J].西南农业学报,2012,25(4):1432-1438. [18]张立金,王晓.基质固相分散-气相色谱-质谱法检测水果中的农药多残留[J].食品科学,2009,30(16):262-265. [19]雷绍荣,郭灵安,毛建霏,等.氯吡脲残留检测技术进展及趋势[J].中国测试,2011,37(6):53-56.
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备注/Memo
第一作者简介：高向阳(1949-)，男，本科，教授，现主要从事食品分析及天然资源研究与开发研究等工作。E-mail：ndgaoxy@163.com.