WANG Wen-jing,WANG Peng,LI Wei-qiang.Study on the Tissue Culture and Rapid Propagation of Lonicera japonica var.chinensis[J].Northern Horticulture,2013,37(18):100-102.
红金银花组培快繁技术研究
- Title:
- Study on the Tissue Culture and Rapid Propagation of Lonicera japonica var.chinensis
- 文章编号:
- 1001-0009(2013)18-0100-03
- 分类号:
- Q 943.1
- 文献标志码:
- A
- 摘要:
- 以红金银花顶芽为试材，利用仿自然气候的培养条件，通过诱导生芽、增殖培养、诱导生根对红金银花进行离体培养，研究了红金银花组培快繁技术体系。结果表明：红金银花适宜的诱导生芽培养基为MS+KT 1.0 mg/L+6-BA 2.0 mg/L+α-NAA 0.1 mg/L，适宜的增殖培养基为MS+KT 1.5 mg/L+6-BA 1.5 mg/L+α-NAA 0.3 mg/L，适宜的生根培养基为1/2MS+α-NAA2.0 mg/L+IAA 0.15 mg/L和1/2MS+α-NAA 2.0 mg/L+IAA 0.20 mg/L。
- Abstract:
- Using the terminal bud of Lonicera japonica var.chinensis as experimental material，the tissue culture and rapid propagation of it were studied by shoot induction,proliferation and rooting under the culture condition of imitating nature climate.The results showed that the suitable culture medium for shoot induction was MS+KT 1.0 mg/L+6-BA 2.0 mg/L+α-NAA 0.1 mg/L；the suitable culture medium for proliferation was MS+KT 1.5 mg/L+6-BA 1.5 mg/L+α-NAA0.3 mg/L；the suitable culture medium for rooting was 1/2MS+α-NAA2.0 mg/L+IAA 0.15 mg/L and 1/2MS+α-NAA 2.0 mg/L+IAA 0.20 mg/L.
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备注/Memo
第一作者简介：王文静（1970-），女，河南新乡人，硕士，副教授，研究方向为植物生理生化。
基金项目：河南省重点科技攻关计划资助项目（112102110035）。
收稿日期：2013-05-28