GONG Qing-fang,ZHOU Hao,WANG Xin-gui,et al.Comparative Study on Quality of Dendrobium officinale from Different Habits[J].Northern Horticulture,2013,37(08):162-165.
不同产地铁皮石斛的品质比较研究
- Title:
- Comparative Study on Quality of Dendrobium officinale from Different Habits
- 文章编号:
- 1001-0009(2013)08-0162-04
- Keywords:
- Dendrobium officinale; D-mannose; polysaccharides; erianin; content
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 为了筛选铁皮石斛优良种质，在广西、安徽、云南、浙江收集不同产地的铁皮石斛，参照2010年版《中国药典》提供的方法，采用苯酚-硫酸法测定多糖含量，柱前衍生化HPLC法测定甘露糖含量，并使用高效液相法测定毛兰素的含量。结果表明：在0.018～0.090 mg范围内，葡萄糖与吸光值线性关系良好，相关系数r=0.9987，不同产地铁皮石斛多糖含量为 25.15%～36.53%；柱前衍生化HPLC法测定D-甘露糖含量专属性强、精确、重现性好，不同产地铁皮石斛甘露糖含量为18.00%～32.35%；不同产地铁皮石斛毛兰素的含量为0～0.0043%。不同产地的铁皮石斛多糖、甘露糖和毛兰素含量差异显著，其中采自云南、浙江的种质在多糖和甘露糖方面有优良的遗传基础，而广西种质的毛兰素含量相对较高。
- Abstract:
- To screen the elite germplasm of Dendrobium officinale，the content of polysaccharides，D-mannose and erianin from different habits were studied in this paper.According to the methods from the Pharmacopeia of the People’s Repubic of China，the content of polysaccharides was determined by phenol-vitriol colorimetric，and using Pre-column derivatization-HPLC measured D-mannose content，and the content of erianin determined by HPLC.The results showed that a good correlation (r = 0.9987) between glucose level and absorbance values at range from 0.018 to 0.090 mg.The content of polysaccharides of Dendrobium officinale ranged from 25.15% to 36.53%.Pre-column derivatization-HPLC method for the determination of D-mannose content was specific，accurate，reproducible.The D-mannose content of Dendrobium officinale ranged from 18.00% to 32.25%.The contents of erianin ranged from 0 to 0.0043%.There were singnificantly difference in the content of polysaccharides，D-mannose and erianin of Dendrobium officinale from different habits.
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备注/Memo
第一作者简介：龚庆芳（1980-），女，博士，助理研究员，研究方向为植物引种栽培及质量评价。E-mail:qingfang_gong@126.com.