YIN Li-hui,HOU Lei-ping,WANG Ting-ting,et al.Study on Optimization of Factors Related to Regeneration of the Tomato[J].Northern Horticulture,2013,37(08):93-96.
番茄子叶再生相关因素的优化研究
- Title:
- Study on Optimization of Factors Related to Regeneration of the Tomato
- 文章编号:
- 1001-0009(2013)08-0093-04
- Keywords:
- tomato; cotyledon; regeneration; elongation
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以“中蔬四号”番茄品种为试材，研究了不同苗龄和不同有机添加物对番茄愈伤组织诱导及芽分化的影响，并筛选了不定芽伸长和生根的最佳培养基。结果表明：子叶苗龄在8～9 d时再生率较高；有机添加物为0.07% MES的MS培养基+ZT 2.0 mg/L+IAA 0.1 mg/L比N+N有机更有利于番茄子叶的再生；不定芽伸长的最佳培养基组合为MS+ZT 1.0 mg/L+GA 1.0 mg/L；而生根的培养基组合为1/2MS+IAA 0.3 mg/L。该研究为今后采用基因工程技术改良番茄性状奠定了基础。
- Abstract:
- Taking Lycopersicum esculentum ‘Zhongshu No.4’ as test material，the effects of different seedling ages and different organic additives on callus induction and bud differentiation were studied.The best medium for adventitious shoot elongation and rooting were screened.The results showed that the regeneration rate was high when the seedling was in the age of 8~9 d；regeneration from the cotyledon cultured in MS with 0.07% EMS as the organic matter+ZT 2.0 mg/L+IAA 0.1 mg/L was better than in MS with N+N organic matter；the best medium for adventitious shoot elongation was MS+ZT 1.0 mg/L+GA 1.0 mg/L，while for rooting was 1/2MS+IAA 0.3 mg/L.This research facilitated the modification of tomato traits by genetic engineering technology in the future.
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备注/Memo
第一作者简介：银利辉（1985-），女，硕士，研究方向为蔬菜育种及生物技术。E-mail:yinlihui258258@126.com.