LI Ling,LI Hou-hua,ZHAO Bing,et al.Pigments Analysis of Malus ‘Royalty’ Red Leaves[J].Northern Horticulture,2013,37(08):71-75.
红色叶王族海棠成色色素分析
- Title:
- Pigments Analysis of Malus ‘Royalty’ Red Leaves
- 文章编号:
- 1001-0009(2013)08-0071-05
- Keywords:
- Malus ‘Royalty’; anthocyanin; cyanidin-galactoside
- 分类号:
- S 687
- 文献标志码:
- A
- 摘要:
- 利用紫外可见分光光度计、薄层层析色谱法、高效液相色谱仪-二极管阵列检测器(HPLC-DAD)对王族海棠叶片色素进行了鉴定,并对鉴定出的单体酚、叶绿素、类胡萝卜素以及总黄酮含量进行定量分析。结果表明:王族海棠叶片内含有叶绿素、类胡萝卜素、矢车菊素半乳糖苷、矢车菊素及其它单体酚(儿茶素、绿原酸、表儿茶素、芦丁、对香豆酸、二氢槲皮素、木犀草素、槲皮素、山奈酚、根皮素、柚皮素)。其中,矢车菊素半乳糖苷和矢车菊素为王族海棠叶片呈现红色的主要色素,叶绿素起到使叶色加深的作用,类胡萝卜素、芦丁、木犀草素、槲皮素、山奈酚、柚皮素等起着辅助成色的作用。
- Abstract:
- Pigments of Malus ‘Royalty’ leaves were qualitative identified using of UV photo-spectrometer,thin layer chromatography and high performance liquid chromatography;and quantitative analysized using of NaNO2-Al(NO3)3-NaOH coloration system and HPLC-DAD.The results showed that Malus ‘Royalty’ leaves contains chlorophyll,carotenoids,cyanidin,cyanidin-galactoside and other monomer phenol(catechin,chlorogenic acid,epicatechin,rutin,ρ-coumaric acid,dihydroquercetin,lueolin,quecetin,kaempferol,phloretin,naringenin).The red color of Malus ‘Royalty’ leaves was caused by accumulation of cyanidin,cyanidin-galactoside and chlorophylls.Carotenoids,rutin,lueolin,quecetin,kaempferol,naringenin played a role as co-pigments.
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备注/Memo
第一作者简介:李玲(1988-),女,宁夏吴忠人,硕士,现主要从事天然产物化学与植物色素分析等研究工作。E-mail:liling20072008@126.com.