QIN Xiu-li,XING Li,YIN Rui.Study on Artificial Cultivation Optimization of Solid Culture Medium of Cordyceps militaris [J].Northern Horticulture,2013,37(06):149-152.
北冬虫夏草人工培养固体培养基优化研究
- Title:
- Study on Artificial Cultivation Optimization of Solid Culture Medium of Cordyceps militaris
- 文章编号:
- 1001-0009(2013)06-0149-04
- Keywords:
- Cordyceps militaris; solid culture; nutrient solution; orthogonal test
- 分类号:
- S 567.3+5
- 文献标志码:
- A
- 摘要:
- 对北冬虫夏草人工培养固体培养基进行了优化。结果表明：北冬虫夏草能够利用的固体培养基的种类较多，在单一的固体培养基质中，以大米为固体基质菌丝长势最好，出草率最高；但在大米中加入10%～15%麦麸，其菌丝长势和出草率都优于单一大米的基质；对营养液进行对比试验及L9(34)的正交实验，确定营养液的适宜配方为蔗糖2.0%，酵母膏1.5%,MgSO4 0.10%，KH2PO4 0.10%；北冬虫夏草固体培养基质的适宜配方为大米85%、麦麸15%,500 mL的装量为60 g，pH值5.5~6.5，料水比（固体基质∶营养液）1∶1.5。
- Abstract:
- Artificial cultivation of solid culture medium of Cordyceps militaris were optimized in this experiment.The results showed that the C.militaris could be cultivated in many types of solid culture medium，the growth rate and density of the hyphae were the highest in a single solid culture medium with rice as the solid substrate，but adding 10%～15% wheat bran in rice solid culture medium，the growth rate and density of the hyphae were better than the single matrix of rice；the suitable nutrient solution was determined by contrast test and L9(34) orthogonal test，the composition was sucrose 2.0%，yeast extract 1.5%，MgSO4 0.10%，KH2PO4 0.10%；the suitable solid culture medium of the C.militaris sinensis was determined by L9(33) orthogonal test，the composition was rice 85%，wheat bran 15%，with 60 g substance in 500 mL bottle,pH 5.5~6.5，the ratio of solid and water (solid matrix/nutrient solution) was 1∶1.5.
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备注/Memo
第一作者简介：秦秀丽（1966-），女，吉林永吉人，硕士，副教授，现主要从事微生物及食药用菌的教学与科研工作。