CAI Ying-hao,GONG Ning,LI De-qin,et al.Changes of Total Phenols and Related Enzymes Activity in the Browning Process of Bletilla striata Protocorm[J].Northern Horticulture,2013,37(06):97-99.
白及原球茎褐变过程中总酚及相关酶活性的变化
- Title:
- Changes of Total Phenols and Related Enzymes Activity in the Browning Process of Bletilla striata Protocorm
- 文章编号:
- 1001-0009(2013)06-0097-03
- Keywords:
- Bletilla striata protocorm; browning; total phenos content; PPO; POD; PAL
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以不同褐变程度的白及原球茎为试材,研究了白及原球茎褐变度与总酚含量、多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性的关系。结果表明:在一定褐变度范围内,随褐变程度的增加,总酚含量升高,超过一定范围,总酚含量随褐变程度增加而降低;PPO与PAL活性变化趋势一致,在褐变前期与褐变度呈正相关,后期活性随褐变度增加而降低;POD活性随褐变度增加而略有降低。
- Abstract:
- Taking Bletilla striata protocorm of varying browning degrees as the basic materials,the relation between the Bletilla striata protocorm browning degree and the activity of PPO,POD and PAL were studied.The results indicated that within some limits,the total phenols went up as the browning degree increased.On the contrary,the total phenols decreased as the browning degree went up over a range.The activity variation of PPO tended to be on accordance with that of PAL,which kept a positive correlation with browning degree in prophase and they went down as browning degree went up at the latter stage.The activity of POD went down slightly as the browning degree increased.
参考文献/References:
[1]
中国植物志编委会
.
中国植物志
[M].18
卷
.
北京
:
科学出版社
,1999:50.
[2]石晶,罗毅波,宋希强.我国白及市场调查与分析[J].中国园艺文摘,2010(8):1751-1755. [3]石云平,李峰,凌征柱.白及组织培养与快繁技术研究[J].广西农业科学,2009,40(11):1408-1410. [4]刑会琴,李敏权,徐秉良,等.过氧化物酶和苯丙氨酸解氨酶与苜蓿白粉病抗性的关系[J].草地学报,2007(4):376-380. [5]许传俊,李玲,李红,等.蝴蝶兰外植体褐变发生与总酚含量、PPO、POD和PAL的关系[J].园艺学报,2006,33(3):671-674. [6]印芳,葛红,彭克勤,等.蝴蝶兰组培褐变与酚酸类物质及相关酶活性的关系[J].中国农业科学,2008,41(7):2197-2203. [7]张宽朝,金青.苯丙氨酸解氨酶与其在重要次生代谢产物调控中的作用研究进展[J].中国农学通报,2008,24(12):59-62. [8]贾慧敏,韩涛,李丽萍,等.可食性涂膜对鲜切桃褐变的影响[J].农业工程学报,2009,25(3):282-286. [9]刘玲玲.植物组织培养中外植体发生褐变的原因及防止措施[J].喀什师范学院学报,2011,3(32):44-46. [10]Soleka D,Kacperska A.Phenylpropaniod deficiency effects the course of plant acclimation to cold[J].Physiologia Plantarum,2003,119:253-262. [11]曾镭,刘燕.植物组织培养中褐化问题的研究进展[J].安徽农学通报,2007,13(14):49-50. [12]江昌俊,余有本.苯丙氨酸解氨酶的研究进展(综述)[J].安徽农业大学学报,2001,28(4):425-430. [13]Hisaminato H,Murata M,Homma S.Relationship between the enzymatic browning and phenylalanine ammonia-lyase activity of cut lettuce,and the prevention of browning by inhibitors of polyphenol biosynthesis[J].Biosci Biotechnol Biochem,2001,65:1016-1021.
相似文献/References:
[1]李树丽.温度及光照条件对中华红叶杨外植体褐变的影响[J].北方园艺,2014,38(04):94.
LI Shu-li.Effect of Temperature and Light Condition on Explants Browning of Populus×euramericanacv.Zhonghuahongye[J].Northern Horticulture,2014,38(06):94.
[2]曾朝珍,张永茂,康三江,等.果蔬褐变抑制机理研究进展[J].北方园艺,2013,37(05):186.
ZENG Chao-zhen,ZHANG Yong-mao,KANG San-jiang,et al.Research Progress of Browning Inhibitory Mechanism in Fruits and Vegetables[J].Northern Horticulture,2013,37(06):186.
[3]蒋钦任,马雅美,马永强,等.软儿梨冷冻贮藏过程生理指标的变化研究[J].北方园艺,2013,37(03):139.
JIANG Qin-ren,MA Ya-mei,MA Yong-qiang,et al.Study on Changes of Related Physiological Index of Ruaner Pear at Frozen Storage Process[J].Northern Horticulture,2013,37(06):139.
[4]周会玲,柏广玲,张欢,等.柠檬汁对梨汁褐变作用的研究[J].北方园艺,2013,37(03):149.
ZHOU Hui-ling,BAI Guang-ling,ZHANG Huan,et al.Study on Lemon Juice on the Browning of Pear Juice[J].Northern Horticulture,2013,37(06):149.
[5]王君,刘福春,李欣,等.不同浓度水杨酸处理对采后鸭梨果实贮藏过程中褐变的影响[J].北方园艺,2012,36(16):151.
WANG Jun,LIU Fu-chun,LI Xin,et al.Effects of Different Salicylic Acid Concentrations Treatments on Postharvest ‘Yali’ Pear During Storage Browning[J].Northern Horticulture,2012,36(06):151.
[6]李新明,张俊,张永茂.无硫复合护色液对“红富士”苹果片干制过程中相关酶活性的影响[J].北方园艺,2013,37(02):108.
LI Xin-ming,ZHANG Jun,ZHANG Yong-mao.Effect of the Color-protection Liquid on Browning-related Enzymes Activities in ‘Red Fuji’ Apple Slices During Drying Process[J].Northern Horticulture,2013,37(06):108.
[7]尹航,陈辉,关军锋.梨果实贮藏中高二氧化碳伤害及其机理的研究进展[J].北方园艺,2014,38(16):185.
YIN Hang,CHEN Hu,GUAN Jun-feng.High CO2 Injury and Its Mechanism in Pear During Storage[J].Northern Horticulture,2014,38(06):185.
[8]林国智.影响香菇组织分离褐变的因素研究[J].北方园艺,2015,39(06):126.[doi:10.11937/bfyy.201506035]
LIN Guo-zhi.Study on Factors Affecting the Browning During the Issue Isolation of Shiitake Mushroom[J].Northern Horticulture,2015,39(06):126.[doi:10.11937/bfyy.201506035]
[9]李磊,涂郑禹,宋翔,等.不同处理对采后“南果梨”贮藏期间果皮钙形态及褐变的影响[J].北方园艺,2016,40(04):128.[doi:10.11937/bfyy.201604033]
LI Lei,TU Zhengyu,SONG Xiang,et al.Effect of Different Postharvest Treatments on Peel Calcium Fractions and Browning of ‘Nan Guo’ Pear Fruit[J].Northern Horticulture,2016,40(06):128.[doi:10.11937/bfyy.201604033]
[10]董晓颖,程斐,李培环,等.白菜小孢子培养中不同因素对玻璃化及褐变形成的影响[J].北方园艺,2016,40(08):85.[doi:10.11937/bfyy.201608024]
DONG Xiaoying,CHENG Fei,LI Peihuan,et al.Effect of Different Factors on the Formation of Vitrification and Browning in the Anther Culture of Chinese Cabbage[J].Northern Horticulture,2016,40(06):85.[doi:10.11937/bfyy.201608024]
备注/Memo
第一作者简介:蔡英豪(1985-),男,硕士,研究方向为应用生物化学。E-mail:caiyinghao0311@163.com.