HUANG Hai-tao,WANG Dan,CHEN Xia.Isozyme Electrophoresis Analysis of Salt-tolerant Mutant of Lily[J].Northern Horticulture,2013,37(03):127-129.
百合耐盐突变体同工酶电泳分析
- Title:
- Isozyme Electrophoresis Analysis of Salt-tolerant Mutant of Lily
- 文章编号:
- 1001-0009（2013）03-0127-03
- Keywords:
- lily; salt-tolerant mutant; isozyme
- 分类号:
- S 682.2+9
- 文献标志码:
- A
- 摘要:
- 以东方百合离体不定芽为试材，通过POD、SOD同工酶电泳技术，研究了不同耐盐性的百合耐盐突变体同工酶的差异性。结果表明：百合的耐盐性与同工酶的表达有一定的相关性，耐盐突变体的POD同工酶谱以及不定芽的SOD同工酶谱与对照比较出现了新的谱带，且随着突变体耐盐能力的增强，突变体植株与对照的同工酶酶谱相似性越来越低，亲缘关系越来越远。
- Abstract:
- Taking Oriental lily in vitro adventitious buds as test material，the difference of isozyme in various salt-tolerance mutants of lily by POD and SOD electrophoresis technique were studied.The results showed that the salt-tolerance of lily had certain relevance with isozyme expression.The mutants had more special new bands of POD isozyme and SOD isozyme than the control.With the increase of the ability of salt tolerance of mutant plants，the isozyme zymogram similarity was more and more low and the phylogenetic relationship was more and more far compared with the control.
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备注/Memo
第一作者简介：黄海涛（1983-），男，湖南新宁人，在读博士，研究方向为园艺植物遗传育种。E-mail：cet_ch@163.com.