WU Xiang-qin,WU Ting-ting,YE Zhen-feng,et al.Study on the Methods Promoting Conidia Formation of Valsa ambiens on Pear Tree[J].Northern Horticulture,2013,37(24):133-135.
梨树腐烂病病菌诱导产孢方法研究
- Title:
- Study on the Methods Promoting Conidia Formation of Valsa ambiens on Pear Tree
- 文章编号:
- 1001-0009(2013)24-0133-03
- Keywords:
- pear canker; conidia; promote conidia formation
- 分类号:
- S 661.2
- 文献标志码:
- A
- 摘要:
- 采用5种培养基质,在不同的光照培养条件下,对梨树腐烂病病菌的产孢情况进行了研究。结果表明:接种腐烂病菌丝的基质在25℃的恒温培养下,经持续黑光灯处理,分生孢子器的产生时间缩短3~5 d,且成熟率提高10%~20%;其中,在30% PBA培养基培养15 d后产生大量黑色分生孢子器,25 d后从分生孢子器体中溢出黄色的分生孢子角;接种病原菌丝的蒲城雪梨树枝条产生的分生孢子器时间较抗病性强的杜梨和秋白梨提前2~4 d,且孢子器和分生孢子数量最多。
- Abstract:
- The formation condition of the conidia was evaluated using five kinds of substrates under the condition of different illumination cultivating.The results showed that the pycnidium producing time was shortened 3~5 days by sustained in UV light and the rate of maturity of pycnidium increased by 10%~20% under 25℃ temperature raised.Among them,the fungi produced a large number of black pycnidium cultured in 30% of the PBA medium under UV light after 15 days.Then the pycnidium extruded conidia threads after 10 days.The pycnidium producing time of the inoculated Puchengxue pear (Pyrus pyrifolia) twigs with V.ambiens was less 2~4 days than the Qiubai pear (P.bretschneideri) which was resistance to weak and the most number of pycnidiums and conidia were produced.
参考文献/References:
[1]王旭丽,康振生,黄丽丽,等.ITS序列结合培养特征鉴定梨树腐烂病菌[J].菌物学报,2007,26(4):517-527. [2]Suzaki K.Population structure of Valsa ceratosperma,causal fungus of Valsa canker,in apple and pear orchards[J].J Gen Plant Pathol,2008,74:128-132.
[3]赵红,王彩霞,陈晓忍,等.苹果腐烂病菌诱导产孢方法[J].中国农学通报,2012,28(10):151-154.
[4]Xin Y F,Shang J J.Bio-control trials of Chaetomium spirale ND35 against apple canker[J].Journal of Forestry Research,2005,16(2):121-124.
[5]Bessho H,Tsuchiya S,Soejima J.Screening methods of apple trees for resistance to Valsa canker[J].Euphytica,1994,77:15-18.
[6]周宗山,徐成楠,吴玉星,等.苹果树腐烂病病菌分生孢子获取方法研究[J].中国果树,2011(2):41-43.
[7]王英,师效欣,杜国强.苹果轮纹病病菌分生孢子离体人工诱导技术研究[J].中国农学通报,2009,25(1):194-197.
[8]陈曲,翟慧者,胡同乐,等.苹果树腐烂病菌分生孢子产生条件研究[J].安徽农业科学,2011,39(25):15337-15338,15351.
[9]冷伟锋,李保华.苹果轮纹病诱导产孢方法[J].植物病理学报,2009,39(5):536-539.
[10]刘振宇,元玲美,王志勇,等.梨树腐烂病病原菌生长特性的研究[J].中国果树,2002(2):7-9.
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备注/Memo
第一作者简介:吴湘琴(1990-),女,硕士研究生,现主要从事植物病害流行和药剂防治筛选等研究工作。E-mail:1040382345@qq.com.
责任作者:朱立武(1961-),男,硕士,教授,研究方向为果树资源与生物技术育种。E-mail:zhuliwu@ahau.edu.cn.
基金项目:国家公益性行业(农业)科研专项资助项目(201203034)。
收稿日期:2013-09-06