WANG Fang,WANG Li-yuan,QIAO Yu,et al.Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System[J].Northern Horticulture,2013,37(24):112-115.
苦瓜MAP30基因的密码子优化及在毕赤酵母中的表达
- Title:
- Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System
- 文章编号:
- 1001-0009(2013)24-0112-04
- Keywords:
- Momordica charantia; MAP30; Pichia pastoris; pGAPHα; optimized gene
- 分类号:
- S 642.5
- 文献标志码:
- A
- 摘要:
- 以苦瓜为试材，采用SDS-PAGE和Western-blot的方法，研究经密码子优化后的苦瓜MAP30(Momordica anti-HIV protein of 30 kDa)基因在新型毕赤酵母中的表达。结果表明：与未优化基因相比，优化基因在新型毕赤酵母中的表达量有所提高。pGAPHα与甲醇诱导型启动子(pPIC9)相比提高了安全性，适合应用到医药领域。
- Abstract:
- Taking Momordica charantia as experimental material，the codon optimized MAP30 gene of Momordica charantia in Pichia pastoris expression was studied by SDS-PAGE and Western-blot.The results showed that the expression quantity of the optimized MAP30 gene was increased slightly in the neotype Pichia pastoris expression system.pGAPHα vector composed of GAP promotor was safer than pPIC9 composed of AOX promotor.It is supposed that the new way should be used in medical field.
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备注/Memo
第一作者简介：王芳（1987-）,女，硕士研究生，研究方向为植物分子生物学。E-mail：291392224@qq.com.
责任作者：丁国华（1963-）,男，博士，教授，研究方向为植物分子生物学。E-mail：hsddgh@hrbnu.edu.cn.
基金项目：黑龙江省教育厅科研资助项目（11551142）;黑龙江省高校科技创新团队研究计划资助项目；哈尔滨师范大学科技创新团队研究计划资助项目（KJTD-2011-2）。
收稿日期：2013-09-16