DONG Jing-chao.Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe’[J].Northern Horticulture,2013,37(24):106-108.
“红颜”草莓茎尖组织培养快繁技术研究
- Title:
- Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe’
- 文章编号:
- 1001-0009（2013）24-0106-03
- 分类号:
- S 668.4
- 文献标志码:
- A
- 摘要:
- 以“红颜”草莓苗为试材，以茎尖为外植体，以MS为基础培养基，研究了最佳激素配比及最佳移栽基质对“红颜”草莓苗生长的影响，以期建立一个高效的茎尖组织培养快繁技术体系。结果表明：适宜的初代培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂5 g/L，萌芽率为81%；适宜的增殖培养基为MS+6-BA 0.25 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂5 g/L，增殖系数为9.1；最适宜的生根培养基为1/2 MS+NAA 0.1 mg/L+蔗糖30 g/L+琼脂5 g/L，生根率为98.0%；最佳移栽基质为营养土∶珍珠岩∶蛭石=1∶1∶1，移栽成活率达到97.7%。
- Abstract:
- Taking the Fragaria×ananassa Duch ‘Benihoppe’ as the experimental material，the shoot tip as explants，MS as the basic medium，the optimal proportion of hormones and best transplanting substrate on growth of strawberry plantlets confidante were studied，in order to establish an efficient system of rapid propagation technique of cultivating a tip of stem tissue.The results showed that the suitable medium for primary MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，germination rate was 81%；suitable proliferation culture medium for MS+6-BA 0.25 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，mutiplication coefficent was 9.1.The most suitable rooting medium was 1/2 MS+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，the rooting rate was 98.00%.The best transplanting matrix∶nutrition soil∶perlite∶vermiculite= 1∶1∶1，transplanting survival rate reached 97.7%.
参考文献/References:
[1]方博云,费鸣毅.草莓新品种—红颜[1].科技致富向导,2005(2):18.
[2]潘忠贵.红颜草莓[J].农业知识,2008(11):13.
[3]晁慧娟,刘敏,姬谦龙,等.‘红颜’草莓茎尖培养与快速繁殖[J].北京农学院学报,2009,10(4):14-16.
[4]宋向丽,吴志慧,陆梦溪，等.正交试验优化红颜草莓离体再生体系[J].安徽农业科学,2012,40（29）：14188-14190.
[5]李志强,王晶,丁国亮,等.草莓热处理结合茎尖脱毒技术研究[J].北方园艺，2012(5):125-127.
[6]董莉,晁慧娟,董清华,等.红颜草莓叶片再生体系的建立[J].中国农学通报,2010,26(10):246-249.
相似文献/References:
[1]李云玲,孙 虎,苗锦山,等.黄山贡菊茎尖脱毒快繁技术研究[J].北方园艺,2014,38(01):106.
LI Yun-ling,SUN Hu,MIAO Jin-shan,et al.Tissue Culture and Rapid Propagation of Chrysanthemum morifolium[J].Northern Horticulture,2014,38(24):106.
[2]杨莉娜,何涛,王海涛.藏药植物川西獐牙菜的快速繁殖技术研究[J].北方园艺,2013,37(13):141.
YANG Li-na,HE Tao,WANG Hai-tao.Rapid Propagation of Tibetan Medicinal Plant Swertia mussotii Franch[J].Northern Horticulture,2013,37(24):141.
[3]王金录,钤泰琳,赵美爱.青岛百合包埋-玻璃化法超低温保存技术研究[J].北方园艺,2012,36(18):135.
WANG Jin-lu,QIAN Tai-lin,ZHAO Mei-ai.Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method[J].Northern Horticulture,2012,36(24):135.
[4]梁美霞.连翘茎尖培养与快速繁殖研究[J].北方园艺,2012,36(18):139.
LIANG Mei-xia.Study on Shoot Tips Culture and Rapid Propagation of Forsythia suspensa[J].Northern Horticulture,2012,36(24):139.
[5]曹冬煦,周海峰,于生成,等.“巨峰”葡萄热处理茎尖培养技术研究[J].北方园艺,2012,36(09):116.
CAO Dong-xu,ZHOU Hai-feng,YU Sheng-cheng,et al.Study on Heat-treated Stem Tip Culture of Kyoho Grape[J].Northern Horticulture,2012,36(24):116.
[6]宋健坤,黄曼娜,王然.梨砧木OH×F51的离体组织培养研究[J].北方园艺,2014,38(18):107.
SONG Jian-kun,HUANG Man-na,WANG Ran.Research on in vitro Tissue Culture of Pear Rootstocks OH×F51[J].Northern Horticulture,2014,38(24):107.
[7]王建书,孙松松,刘浩,等.西葫芦茎尖离体培养和快速繁殖技术[J].北方园艺,2015,39(22):96.[doi:10.11937/bfyy.201522025]
WANG Jianshu,SUN Songsong,LIU Hao,et al.Induction of Direct Shoot Organogenesis From Shoot Tip Explants of Cucurbita pepo L.[J].Northern Horticulture,2015,39(24):96.[doi:10.11937/bfyy.201522025]
[8]张艳秋,屈连伟,苏君伟,等.菊花茎尖玻璃化法超低温保存技术研究[J].北方园艺,2016,40(05):128.[doi:10.11937/bfyy.201605034]
ZHANG Yanqiu,QU Lianwei,SU Junwei,et al.Study on Cryopreservation of Shoot Tips From in vitro Plants of Chrysanthemum by Vitrification Method[J].Northern Horticulture,2016,40(24):128.[doi:10.11937/bfyy.201605034]
[9]马少梅,麻冬梅,谢应忠,等.北海道黄杨茎尖组织培养初报[J].北方园艺,2010,34(03):0.[doi:10.11937/bfyy.201003035]
MA Shao-mei,MA Dong-mei,XIE Ying-zhong,et al.Advances on the Establislunent of Shoot-tip of Euonymus japonicus[J].Northern Horticulture,2010,34(24):0.[doi:10.11937/bfyy.201003035]
备注/Memo
作者简介：董敬超（1979-），女，硕士，助理研究员，现主要从事植物组织培养与生态农业实验室常规化验等研究工作。E-mail:djchlt@163.com.
收稿日期：2013-09-09