[1]崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响[J].北方园艺,2013,37(24):31-34.
　CUI Lu-lu,YU Hai-xia,LI Jing-fu,et al.Effect of PEG on Seed Vigor of Aging Cabbage[J].Northern Horticulture,2013,37(24):31-34.
点击复制

聚乙二醇对老化甘蓝种子活力的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年24期 页码: 31-34 栏目: 出版日期: 2013-12-30
Title:
Effect of PEG on Seed Vigor of Aging Cabbage
文章编号:
1001-0009(2013)24-0031-04
作者:
崔路路; 于海霞; 李景富; 许向阳
东北农业大学 园艺学院，黑龙江 哈尔滨 150030
Author(s):
CUI Lu-lu; YU Hai-xia; LI Jing-fu; XU Xiang-yang
College of Horticulture，Northeast Agricultural University，Harbin，Heilongjiang 150030
关键词:
甘蓝; PEG; 人工老化; 自然老化; 种子活力
Keywords:
cabbage; PEG; artificial aging; natural aging; seed vigor
分类号:
S 565.401
文献标志码:
B
摘要:
以“晚丰”甘蓝种子为试材，采用不同分子量、浓度、处理时间的PEG渗透剂分别对自然老化和人工老化种子进行组合引发处理，比较了不同PEG处理组合对老化甘蓝种子可溶性蛋白质含量、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性的影响。结果表明：PEG对老化种子有一定修复作用，可以提高种子活力；PEG分子量因素对自然老化种子的影响最大，浓度次之，处理时间最小；而经PEG渗透调节后老化种子萌发代谢过程有所改变，其中MDA含量比对照降低，可溶性蛋白质含量有所提高，抗氧化酶系统活性高于对照组；同时因老化条件的不同，自然老化与人工老化在生理生化方面存在差异，对PEG引发反应也有所区别，自然老化种子PEG引发效果较明显。
Abstract:
Taking ‘Wanfeng’ cabbage seeds as material，PEG penetrant combination treatment with different molecular weight，concentration，treatment time were adopted to natural aging and artificial aging of seeds，the effects of different combination of PEG treatment on soluble protein content，malondialdehyde (MDA) content，superoxide dismutase (SOD)，catalase (CAT) and peroxidase (POD) activity were compared.The results showed that PEG had some repair effect on aging seed，could improve seed vigor.PEG molecular had significant influence on natural aging seeds，followed by was the factors of PEG concentration，the minimum was PEG treatment time.Aging seed metabolic germination had changed after PEG-osmotic processes，including MDA content was lower than control，soluble protein content increased，antioxidant enzymes activity was higher than CK.At the same time due to aging conditions，there were difference on physiological and biochemical between the natural aging and artificial aging seeds，including the PEG induced reactions，natural aging seeds had the most obvious induced reactions after PEG triggered.

参考文献/References:

[1]杨建平,唐玉林.小麦种子衰老的生理生化分析[J].种子,1955(2):13-14.

[2]颜启传.种子活力的测定方法原理与技术[M].北京:中国农业出版社,2005.
[3]孔治有,刘叶菊,覃鹏.人工老化处理对小麦种子生理生化特性的影响[J].亚热带植物科学，2011,39(1):17-20.
[4]Heydecker W ,Higgins J,Gulliver R L.Accelerated germination by osmotic seed treatment [J].Nature,1973,246:42-44.
[5]Woodstoek L W，Tao K L.Preventlon of imbibitional injury in low vigor soybean Embryonic axes by osmotic control of water uptake[J].Physiologia Plantarum,1981,51:133-139.[6]崔秀敏,王秀峰,杜宏斌.PEG引发种子的研究进展[J].塔里木农垦大学学报,2000(12):4.
[7]王慧超,何士敏,李昌满.PEG渗调处理对植物种子的影响[J].安徽农业科学,2008，36(6):2224-2226.
[8]郑晓鹰,任祝三.测定种子活力方法之探讨[J].种子,1982(4):31-34.
[9]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000:164-168.
[10]张晓艳,李宇歌.PEG渗透处理对老化种子活力的影响[J].吉林师范大学学报(自然科学版),2005,5(2):50-52.
[11]吴道藩,宋明.提高甘蓝种子活力的方法与机理研究[J].园艺学报,2002,29(6):524-526.
[12]Delouche J C,Baskin C C.Accelerated aging techniques for predicting the relative storability of seed lots[J].Seed Science and Technology,1973(3):427-452.
[13]Basra S M A，Ahmad N，Khan M，et al.Assessment of cotton deterioration during accelerated aging[J].Seed Science and Technology,2003,31(3):531-540
[14]Machado N B，Custdio C C，Takaki M.Evaluation of naturally and artificially aged seed of Passels vulgarism[J].Seed Science and Technologyb，2001,29(1):137-149.
[15]李稳香,颜启传.杂交水稻自然老化种子与人工老化种子性能差异研究[J].杂交水稻,1997,12(3):26-28.
[16]邹德曼.作物种子物理学[M].杭州：浙江农业大学出版社，1984：99-115.

相似文献/References:

[1]张 爽,赵 纯,董 然,等.槭叶草对PEG-6000模拟干旱胁迫的生理响应[J].北方园艺,2013,37(22):74.
　ZHANG Shuang,ZHAO Chun,DONG Ran,et al.Physiological Responses of Mukdenia rossii Under PEG-6000 Drought Stress[J].Northern Horticulture,2013,37(24):74.
[2]马志峰,张恩惠,王智民.角额壁蜂对网室制种甘蓝授粉效果的影响[J].北方园艺,2013,37(13):29.
　MA Zhi-feng,ZHANG En-hui,WANG Zhi-min.Pollination Effects of Osmia cornifrons on Breeding Cabbage in Net Houses[J].Northern Horticulture,2013,37(24):29.
[3]高海娜,王朝阳,张恩慧.甘蓝胚状体诱导成苗因素研究[J].北方园艺,2014,38(01):97.
　GAO Hai-na,WANG Zhao-yang,ZHANG En-hui.Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)[J].Northern Horticulture,2014,38(24):97.
[4]高营营,王 超,张智杰.甘蓝CMS不育系和保持系的电镜观察[J].北方园艺,2014,38(02):15.
　GAO Ying- ying,WANG Chao,ZHANG Zhi jie.Electron Microscopic Observation on Male Sterile CMSPM and Maintainer Line of Cabbage[J].Northern Horticulture,2014,38(24):15.
[5]崔路路,于海霞,李景富,等.磁场处理对结球甘蓝种子活力及萌发进程的影响[J].北方园艺,2014,38(02):20.
　CUI Lu- lu,YU Hai- xia,LI Jing- fu,et al.Effect of Magnetic Treatment on Seed Vigor and Seed Germination of Cabbage[J].Northern Horticulture,2014,38(24):20.
[6]金晓霞,于海凤,于丽杰.农杆菌介导的人白细胞介素-4基因转化甘蓝的研究[J].北方园艺,2013,37(15):113.
　JIN Xiao-xia,YU Hai-feng,YU Li-jie.Study on Agrobacterium mediated Inter leukin4 Gene Transferred into Cabbage[J].Northern Horticulture,2013,37(24):113.
[7]郭国寿.甘蓝品种比较试验[J].北方园艺,2012,36(15):38.
　GUO Guo-shou.Comparison Test of Cabbage Varieties[J].Northern Horticulture,2012,36(24):38.
[8]张玮滢,于丽杰.转il-4基因甘蓝后代的遗传及表现分析[J].北方园艺,2012,36(08):133.
　ZHANG Wei-ying,YU Li-jie.Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea[J].Northern Horticulture,2012,36(24):133.
[9]蔡 霞,李桂莲,孟平红,等.大蒜间套菜用糯玉米、夏秋大白菜间套甘蓝一年两季四收栽培模式[J].北方园艺,2015,39(03):52.[doi:10.11937/bfyy.201503016]
　[J].Northern Horticulture,2015,39(24):52.[doi:10.11937/bfyy.201503016]
[10]杨春雪,李丽丽,田波林.不同栽培基质、不同浓度GA3和PEG溶液处理对石刁柏种子发芽的影响[J].北方园艺,2015,39(05):1.[doi:10.11937/bfyy.201505001]
　YANG Chun-xue,LI Li-li,TIAN Bo-lin.Effect of Different Culture Substrates，Different Concentrations of GA3 and PEG on the Germination Characteristics of Asparagus officinalis[J].Northern Horticulture,2015,39(24):1.[doi:10.11937/bfyy.201505001]

备注/Memo

第一作者简介：崔路路（1987-），男，硕士研究生，研究方向为蔬菜遗传育种研究。Email:neaucui@163.com.

责任作者：许向阳(1969-)，男，博士，研究员，博士生导师，研究方向为蔬菜遗传育种及生物技术和蔬菜种质资源。E-mail：xxy709@126.com.
基金项目：国家现代农业产业技术体系专项资金资助项目（CARS-25）；黑龙江省杰出青年科学基金资助项目（JC201204）；哈尔滨市科技创新人才研究专项资金资助项目（2011RFXXN031）；东北农业大学创新团队基金资助项目；东北农业大学博士启动基金资助项目（2009RC09）。
收稿日期：2013-09-03

更新日期/Last Update: 2014-12-06