XIANG Cheng-gang,WANG Yan-ling,ZHANG Xue-mei,et al.Application of SSR Markers in Genetic Relationships Analysis of Cucurbita[J].Northern Horticulture,2013,37(15):104-108.
SSR标记在南瓜亲缘关系分析中的应用
- Title:
- Application of SSR Markers in Genetic Relationships Analysis of Cucurbita
- 文章编号:
- 1001-0009（2013）15-0104-05
- Keywords:
- Cucurbita; SSR; clustering analysis; genetic distance
- 分类号:
- S 642.1
- 文献标志码:
- A
- 摘要:
- 以20份分属10个品种群的中国南瓜、印度南瓜和美洲南瓜材料及1份印度南瓜和中国南瓜的种间杂交种为试材，采用390对SSR引物对21份材料进行了多态性及亲缘关系分析。结果表明：供试材料之间具有丰富的遗传多样性，美洲南瓜与中国南瓜亲缘关系较近，二者与印度南瓜的亲缘关系较远；同一亚种内及同一品种群内材料之间具有更近的亲缘关系；利用SSR分子标记做出的聚类结果基本可以将供试材料按照不同品种群分开。
- Abstract:
- Taking twenty cucurbita materials including C.moschata,C.maxima and C.pepo belonging to 10 cultivar groups and one interspecific hybrid between C.maxima and C.moschata,their polymorphism and genetic relationships were studied using 390 SSR markers.The results showed that the level of polymorphism within cultivals was very high,there were general similarity between the cultival groups methods and molecular methods.The relation between C.pepo and C.moschata was closer than that between C.moschata and C.maximaby clustering.
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备注/Memo
第一作者简介：向成钢（1987-），男，硕士研究生，研究方向为蔬菜育种。E-mail：z0501080535@126.com.
责任作者：王长林（1969-），男，本科，副研究员，现主要从事南瓜遗传育种及相关技术研究工作。
基金项目：国家科技支撑计划资助项目(2012BAD02B03)；中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项资助项目(2060302-2-12)；农业部园艺作物生物学与种质创制重点实验室资助项目。
收稿日期：2013-04-15