[1]林振亚,高必达,赵廷昌.西瓜嗜酸菌pilL菌毛基因的序列分析[J].北方园艺,2013,37(13):121-124.
　LIN Zhen-ya,GAO Bi-da,ZHAO Ting-chang.Sequence Analysis of pilL Gene of Acidovorax citrull[J].Northern Horticulture,2013,37(13):121-124.
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西瓜嗜酸菌pilL菌毛基因的序列分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年13期 页码: 121-124 栏目: 出版日期: 2013-07-15
Title:
Sequence Analysis of pilL Gene of Acidovorax citrull
文章编号:
1001-0009(2013)13-0121-04
作者:
林振亚1; 高必达1; 赵廷昌2
1.湖南农业大学 生物安全科学技术学院，湖南 长沙 410128；
2.中国农业科学院 植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193
Author(s):
LIN Zhen-ya1; GAO Bi-da1; ZHAO Ting-chang2
1.College of Biosafety Science and Technology，Hunan Agricultural University，Changsha，Hunan 410128；
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193
关键词:
西瓜嗜酸菌; pilL基因; 生物信息学分析
Keywords:
Acidovorax citrulli; pilL gene; bioinformatics analysis
分类号:
S 651
文献标志码:
A
摘要:
在西瓜嗜酸菌标准菌株AAC00-1全基因组序列中选取了1个菌毛基因pilL序列，利用这个基因设计引物，选取国内外比较有代表性的7株西瓜嗜酸菌菌株进行PCR扩增，并对PCR产物进行了测序，从生物信息学的角度对这7个菌株pilL基因的序列特征、同源性、分子聚类、信号肽、疏水性、跨膜结构和二级蛋白结构进行了预测分析。结果表明：Aac5、pslbtw37、W1和pslbtw22 pilL基因之间的核苷酸氨基酸特征相近，同源性高，AAC001、AAC20350和Fc376之间亦如此。预测发现7个编码蛋白表现为亲水性，都无跨膜结构，Aac5、pslbtw37和W1 pilL基因都存在一个类型为SPI的信号肽，其分泌蛋白所到达的亚细胞位点为s(即分泌到周质空间)。
Abstract:
The sequence of a pilL gene (locus tag Aave_2768 in whole genome) in standard strain AAC001was downloaded and a pair of primers were designed based on it，the gene encodes a putative type IV pilus protein7 representative strains of Acidovorax citrulli were used to carry out amplification and PCR fragments were sequenced.The comprehensive bioinformatics analyses including sequence characteristics of DNA and amino acid，identity and homology，molecular clustering，protein physicochemical properties，hydrophobicity，transmembrane helices，signal peptide and protein secondary structural prediction were performed.The results showed that the characteristics of pilL at nucleotide and amino acid level were similar among Aac5,pslbtw37,W1 and pslbtw22，so were AAC00-1，AAC203-50 and Fc376.There were not transmembrane helices on 7 coding protein，but find hydrophilic regions on them.A signal peptide (SPI)were found with Aac5，pslbtw37，W1，and codingprotein were secreted into the perilasmic space.

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相似文献/References:

[1]安 心,王铁霖,杨玉文,等.西瓜嗜酸菌分泌系统的研究进展[J].北方园艺,2014,38(14):196.
　AN Xin,WANG Tie-lin,YANG Yu-wen,et al.Research Advances on Secretion Systems in Acidovorax citrulli [J].Northern Horticulture,2014,38(13):196.

备注/Memo

第一作者简介：林振亚（1988-），女，浙江丽水人，硕士研究生，研究方向为分子植物病理学。E-mail:huanglongya@yahoo.cn.

责任作者：赵廷昌（1964-），男，博士，研究员，博士生导师，现主要从事植物细菌病害等研究工作。E-mail:tingchangzhao@gmail.com.
基金项目：国家西甜瓜产业技术体系专项资助项目（CARS-36）；国家公益性行业（农业）科研专项资助项目（201003066）。
收稿日期：2013-03-07

更新日期/Last Update: 2014-08-08