CHEN Xi,WANG Rui,DING Guo-hua.Methods and Progress on Plant DNA Methylation[J].Northern Horticulture,2013,37(12):188-191.
植物DNA甲基化研究方法及进展
- Title:
- Methods and Progress on Plant DNA Methylation
- 文章编号:
- 1001-0009(2013)12-0188-04
- Keywords:
- DNA; methylation; progress; method; MSAP
- 分类号:
- Q 943
- 文献标志码:
- A
- 摘要:
- 该文在详细介绍DNA甲基化机制、原理与研究方法的基础上，概述了国内外有关DNA甲基化研究的热点，分析了盐胁迫、氮处理、重金属处理、热胁迫、干旱胁迫等不同胁迫条件下同种植物间基因的差异性表达和RNA介导的DNA甲基化和DNA去甲基化等；阐述了多种检测植物DNA甲基化水平的技术，尤其对MSAP技术的基本原理、基本程序和应用实例进行了较为细致的介绍；并对未来植物DNA甲基化研究进行了展望。
- Abstract:
- On the basis of a detail introduction of the mechanism，theory and methods of DNA methylation，the important issues about DNA methylation at home and abroad were described；genes differentially expressed of the same species and DNA methylation and DNA demethylation of RNA-mediated under different stress conditions of salt stress，N treatment，heavy metal treatment，heat and drought stress and so on were analyzed.A variety of detection technologies of plant DNA methylation level were introduced，especially on the basic theory，procedure and examples of MSAP；the plant DNA methylation was also prospected.
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备注/Memo
第一作者简介：陈曦（1987-），女，黑龙江哈尔滨人，硕士研究生，研究方向为植物学分子生物学。E-mail:water_drinkme@163.com.’
责任作者：丁国华（1963-），男，山东梁山人，教授，研究方向为植物分子生物学。E-mail：hsdddgh@hrbnu.edu.cn.
基金项目：国家自然科学基金重点资助项目（30830027）；黑龙江省高校科技创新团队研究计划资助项目（KJTD-2011-2）；哈尔滨师范大学科技发展预研计划资助项目。
收稿日期：2013-01-04