LIN Yan-ru,CAI Kai-yu.Study on the Best Extraction Technology of Leaf Protein from Corchorus olitorius L.[J].Northern Horticulture,2013,37(12):144-147.
菜用黄麻叶蛋白最佳提取工艺研究
- Title:
- Study on the Best Extraction Technology of Leaf Protein from Corchorus olitorius L.
- 文章编号:
- 1001-0009(2013)12-0144-04
- 分类号:
- TS 255.36
- 文献标志码:
- A
- 摘要:
- 以菜用黄麻叶为试材,采用酸提法和加热法制备提取液,在料液比、浸提剂pH值、打浆时间、浸提时间等单因素试验基础上,进行L9(34)正交实验设计,研究了提取菜用黄麻叶蛋白的最佳工艺条件。结果表明:菜用黄麻叶蛋白最佳提取工艺条件为:浸提剂pH 5,打浆时间3 min,料液比1∶8,浸提时间6 min;叶蛋白的最佳分离条件为:温度85℃时,沉淀分离出叶绿体蛋白质,调节提取液pH 3和11时分别分离出细胞质蛋白质Ⅰ、Ⅱ。菜用黄麻叶蛋白的提取率为8.07%,得率为37.59%。
- Abstract:
- Taking the leaves of Corchorus olitorius L.as material,on the basis of single factor tests of solid-liquid ratio,pH values of the extracting solvents,pulping time and soaking time,L9(34) orthogonal experiment was used to optimize the extraction conditions of leaf protein concentrate (LPC) using acid method and heating method.The results showed that the best extraction technology was solid-liquid ratio 1∶8,pH values of the extracting solvents 5,pulping time 3 min,soaking time 6 min.The best optimum precipitating conditions were heating temperature 85℃ for the chloroplastic proteins,the pH 3 for cytoplasmic proteins and the pH 11 for the cytoplasmic proteins Ⅱ.Under these conditions,the extraction rate of LPC reached 8.07% and the yield rate was 37.59%.
参考文献/References:
[1]柳青海,张唐伟,李天才.叶蛋白提取分离及应用研究进展[J].食品工业科技,2011,32(9):468-470.
[2]李燕,龚友才,陈基权,等.菜用黄麻嫩梢营养成分测定与分析[J].中国蔬菜,2010(14):67-70.
[3]巨艳,杜娟,高晓磊,等.墨绿海的有效成分及其应用前景分析[J].西北林学院学报,2009,24(5):132-134.
[4]杨月欣,王光亚.实用食物营养成分分析手册[M].北京:中国轻工业出版社,2002:75-163.
[5]马丹.凯氏定氮法测定食品中蛋白质含量[J].计量与测量技术,2008,35(6):57-58.
[6]大连轻工业学院,华南理工大学,郑州轻工业学院,等.食品分析[M].北京:中国轻工业出版社,2007:221-223. [7]陶健,刘邻渭,毕磊.双缩脲反应快速测定蛋白质的方法学研究[J].食品科技,2004(1):77-79.
[8]王桃云,王金虎.桑叶叶蛋白提取工艺的研究[J].食品研究与开发,2006,27(2):79-81.
[9]周建建,单宇,郑玉红,等.繁缕叶蛋白提取工艺研究[J].食品科学,2009,30(6):109-112.
[10]王娜,褚衍亮,李静,等.樟树叶蛋白提取工艺及四季含量动态分析[J].北方园艺,2011(7):51-54.
相似文献/References:
[1]林燕如,张晓芝.苋菜叶蛋白提取工艺研究[J].北方园艺,2013,37(18):139.
LINYan-ru,ZHANGXiao-zhi.StudyontheExtractionTechnologyofAmaranthusmangostanusL. LeafProteinConcentrate[J].Northern Horticulture,2013,37(12):139.
[2]李初英,黄其椿,赵洪涛,等.富硒高钙保健型帝皇麻菜新品种“桂麻菜1号”的选育[J].北方园艺,2015,39(03):140.[doi:10.11937/bfyy.201503041]
LI Chu-ying,HUANG Qi-chun,ZHAO Hong-tao,et al.A New Variety of Rich Selenium and High Calcium Emperor Vegetable Jute ‘Vegetable Jute of Gui No.1’[J].Northern Horticulture,2015,39(12):140.[doi:10.11937/bfyy.201503041]
[3]赵艳红,侯文焕,唐兴富,等.菜用黄麻对硒的累积规律[J].北方园艺,2018,42(09):73.[doi:10.11937/bfyy.20172513]
ZHAO Yanhong,HOU Wenhuan,TANG Xingfu,et al.Accumulation Rule of Selenium in Vegetable-used Jute (Corchorus olitorius L.)[J].Northern Horticulture,2018,42(12):73.[doi:10.11937/bfyy.20172513]
备注/Memo
第一作者简介:林燕如(1982-),女,广东揭阳人,硕士,实验师,现主要从事基础化学研究与食品分析等工作。E-mail:lyrhuaxue@163.com.
基金项目:韩山师范学院青年基金资助项目(LQ200812)。
收稿日期:2013-01-17