WANG Jing,LI Jing,JIA Ling-yun,et al.Study on the Callus Induction from Epimedium koreanum Nakai[J].Northern Horticulture,2013,37(12):106-108.
朝鲜淫羊藿愈伤组织诱导研究
- Title:
- Study on the Callus Induction from Epimedium koreanum Nakai
- 文章编号:
- 1001-0009(2013)12-0106-03
- Keywords:
- Epimedium koreanum Nakai; callus; induction
- 分类号:
- S 503.53
- 文献标志码:
- A
- 摘要:
- 以朝鲜淫羊藿幼叶、叶柄和茎尖为外植体，研究了朝鲜淫羊藿愈伤组织诱导的适宜条件，以期为工厂化育苗提供科学根据。结果表明：朝鲜淫羊藿愈伤组织诱导最佳外植体为茎尖，接种于培养基MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L+NAA 1.0 mg/L中，于25℃黑暗条件下培养1周后转入光照强度2 000 lx条件下，愈伤组织诱导效果最佳。
- Abstract:
- Taking the young leaf，stem apex and petiolesuitable of Epimedium koreanum Nakai as explants，the condition of its callus induction was studied，in order to provide scientific basis for large scale production of tissue culture.The results showed that the stem apex was the best production material of tissue culture.The optimum medium for callus induction was MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L+NAA 1.0 mg/L.In the temperature of 25℃，the induction effect was good under the cultured conditions of dark，and taken out，then cultured in the light intensity 2 000 lx.
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备注/Memo
第一作者简介：王晶（1977-），女，博士，讲师，现主要从事中药资源研究和开发工作。E-mail:wangjingyk@126.com.
责任作者：路金才（1971-），男，博士，教授，博士生导师，现主要从事中药资源研究和开发等工作。E-mail:jincailu@yahoo.com.cn.
基金项目：国家自然科学基金资助项目（81102755）。
收稿日期：2013-03-07