ZHANG Bo,LIU Cui-jing,LIU Xia.RAPD Analysis on the Genetic Diversity of Genus Rabdosia in Northeast China[J].Northern Horticulture,2013,37(12):87-90.
东北地区香茶菜属植物遗传多样性的RAPD分析
- Title:
- RAPD Analysis on the Genetic Diversity of Genus Rabdosia in Northeast China
- 文章编号:
- 1001-0009(2013)12-0087-04
- 分类号:
- Q 949.95
- 文献标志码:
- A
- 摘要:
- 以东北地区不同产地的24份蓝萼香茶菜和尾叶香茶菜样品为试材，采用RAPD分子标记技术对其进行遗传多样性分析。结果表明：从60条随机引物中筛选出5条引物用于2种香茶菜的扩增，2种香茶菜共扩增得到63条清晰条带，其中55条为多态性条带，多态性比率为87.30%。系统聚类分析结果表明，2种香茶菜亲缘关系明显，遗传多样性较丰富，且种间大于种内。因此，RAPD分子标记技术可以作为研究香茶菜属植物遗传多样性的有效标记方法。
- Abstract:
- Taking 24 samples of Rabdosia japonica (Burm.f.) Hara var.glaucocalyx (Maxim.) Hara and Rabdosia excise (Maxim.) Hara from different regions of northeast China as materials，the genetic diversity was studied using random amplified polymorphism DNA (RAPD) markers.The results showed that 5 primers were selected from 60 primers.63 DNA bands were amplified from the two species of Rabdosia，the percentage of polymorphism was 87.30%.Analysis of Nei’s unbiased genetic distance showed that the genetic relationship between the two species of Rabdosia was significant.The genetic diversity was high，and the genetic diversity of interspecies was higher than that of interspecies.In conclusion,RAPD molecular marker technology could be an efficient method to study the genetic diversity of Rabdosia plants.
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相似文献/References:
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MA Yin -bo,LIU Jiu -shi,HANG Pin -pin,et al.Study on Dedifferentiation and Regeneration Culture of Cotyledon Tissue in Plectranthus japonica ( Burm. ) Koidz. var. glaucocalyx ( Maxim. )Kiodz[J].Northern Horticulture,2011,35(12):0.[doi:10.11937/bfyy.201109054]
[2]汤晓韵,卢曦,白露,等.尾叶香茶菜和蓝萼香茶菜叶的解剖结构[J].北方园艺,2018,42(22):98.[doi:10.11937/bfyy.20173850]
TANG Xiaoyun,LU Xi,BAI Lu,et al.Anatomical Structure of the Leaves of Rabdosia excisa (Maxim.) Hara and Rabdosia japonica (Burm.f.) Hara var.glaucocalyx (Maxim.) Hara[J].Northern Horticulture,2018,42(12):98.[doi:10.11937/bfyy.20173850]
备注/Memo
第一作者简介：张博（1987-），女，吉林松原人，硕士研究生，现主要从事药用植物资源学和生态学研究工作。E-mail:zb1987523@qq.com.
责任作者：刘霞（1965-），女，吉林长春人，博士，副教授，现主要从事中药资源的分类和调查与栽培技术的研究工作。E-mail:Liuxia651015@163.com.
收稿日期：2013-01-17