[1]尹智慧,宋丽莉,司亮,等.大岩桐叶片再生体系的建立及卡那霉素敏感性研究[J].北方园艺,2013,37(10):100-102.
　YIN Zhi-hui,SONG Li-li,SI Liang,et al.Study on Optimization of Tissue Culture System of Sinningia speciosa Benth. and the Sensitivity on Kanamycin[J].Northern Horticulture,2013,37(10):100-102.
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大岩桐叶片再生体系的建立及卡那霉素敏感性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年10期 页码: 100-102 栏目: 出版日期: 2013-06-15
Title:
Study on Optimization of Tissue Culture System of Sinningia speciosa  Benth. and  the Sensitivity on Kanamycin
文章编号:
1001-0009(2013)10-0100-03
作者:
尹智慧; 宋丽莉; 司亮; 于典司; 郭长虹
哈尔滨师范大学 生命科学与技术学院，黑龙江省分子细胞遗传与遗传育种重点实验室，黑龙江 哈尔滨150025
Author(s):
YIN Zhi-hui; SONG Li-li; SI Liang; YU Dian-si; GUO Chang-hong
Key laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang，College of Life Science and Technology，Harbin Normal University，Harbin，Heilongjiang 150025
关键词:
大岩桐; 组织培养; 卡那霉素
Keywords:
Sinningia speciosa ;  Benth.; tissue culture; kanamycin
分类号:
S 682.2+9
文献标志码:
A
摘要:
以大岩桐(Sinningia speciosa Benth.)幼嫩叶片为外植体，研究了不同激素组合对大岩桐叶片再生体系的影响及抗生素卡那霉素的敏感浓度。结果表明：不同激素组合对不定芽的诱导效果差异显著，叶片不定芽的诱导和增殖的最佳培养基均为MS+1.0 mg/L 6-BA+0.1 mg/L NAA，生根适宜培养基1/2MS+0.5 mg/L NAA，大岩桐叶片对卡那霉素的敏感浓度为200 mg/L。
Abstract:
Taking the leaves of Sinningia speciosa  Benth. as explants，the concentration of the different hormone combinations of the Sinningia speciosa Benth. leaves regeneration system and the antibiotics sensitive to kanamycin were studied.The results showed that the significantly different effects of different hormone combinations induction of adventitious bud，the optimal medium for leaf induction was MS with 1.0 mg/L 6-BA and 0.1 mg/L NAA，while the 1/2 MS medium with 0.5 mg/L NAA was successful for rapid rooting.The leaves of Sinningia speciosa  Benth. with 200 mg/Lkanamycin were sensitive.

参考文献/References:

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备注/Memo

第一作者简介：尹智慧(1987-)，女，硕士，研究方向为植物基因工程。E-mail:ly_whly@yahoo.cn.
责任作者：郭长虹(1968-)，女，博士，教授，现主要从事植物基因工程等研究工作。E-mail:kaku2008@hotmail.com.
基金项目：黑龙江省杰出青年基金资助项目(JC201109)；黑龙江省创新团队科研资助项目(2010TD10)；哈尔滨师范大学创新团队资助项目(KJTD201102)。
收稿日期：2013-02-10

更新日期/Last Update: 2014-08-15