LI Hong-jun.Study on Extraction of Flavonoids from Ginkgo biloba Leaves and Antioxidant Action in vitro[J].Northern Horticulture,2013,37(05):156-159.
银杏叶中黄酮类化合物的提取及其体外抗氧化活性研究
- Title:
- Study on Extraction of Flavonoids from Ginkgo biloba Leaves and Antioxidant Action in vitro
- 文章编号:
- 1001-0009(2013)05-0156-04
- Keywords:
- Ginkgo biloba eaves; flavonoids; antioxidation; orthogonal test
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 以银杏叶为原料，研究了单因素（液料比、浸提pH、提取时间、提取温度）对银杏叶中黄酮提取率的影响，并以硼砂-氢氧化钙-水为溶剂提取银杏叶中的黄酮，用正交实验方法优化了银杏叶中黄酮的提取工艺，用油脂抗氧化试验研究了黄酮的抗氧化性。结果表明：影响黄酮提取因素顺序为：提取温度>液料比>提取时间=浸提液pH，最佳提取工艺组合为：A1B2C2D2，即液料比为1∶30，浸提液pH为8，提取温度为80℃，提取时间为3 h，在此工艺条件下银杏叶中黄酮的提取率为2.67%。银杏叶中的黄酮对猪油的氧化具有明显的抑制作用。随着提取物浓度的增加，抗氧化性初期表现为逐渐增强的趋势，当添加量为0.20%时达到最佳抗氧化效果，其抗氧化性不如抗氧化剂维生素C；当提取物添加量继续增大时，抗氧化效果有所下降。
- Abstract:
- Taking the leaves of Ginkgo biloba as material,the single factor (solid-liquid ratio，extraction time，extraction temperature，extraction pH) on extraction rate of flavonoids from Ginkgo biloba were studied，using borax-calcium hydroxide water as solvent extraction，and the extraction methods were optimized by orthogonal test；the antioxidation were studied by oil test.The results showed that the effect of flavone content from Ginkgo biloba leaves were in the order：extraction temperature>ratio of liquor to material>extraction time=extraction pH，optimum extraction technology for A1B2C2D2，namely combination of liquid ratio was 1∶30，extraction pH 8，extraction temperature 80℃，the extraction time was 3 h，under the conditions of flavone in Ginkgo bilobaextraction rate was 2.67%.Ginkgo biloba flavonoid on lard oxidation had obvious inhibition effect.With the increasing concentrations of antioxidant properties of extracts，the initial presentation of gradually increasing，when adding 0.20% to achieve the best antioxidant effect，the oxidation resistance of vitamin C as antioxidants；when the extract was added volume continued to increase，the antioxidant effect declined.
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备注/Memo
作者简介：李红军（1968-），男，本科，农艺师，现主要从事农业技术推广工作。
收稿日期：2012-11-19