[1]单广福,张晓丽,李明军,等.金银花愈伤组织的诱导及继代培养研究[J].北方园艺,2013,37(05):106-109.
　SHAN Guang-fu,ZHANG Xiao-li,LI Ming-jun,et al.Study on Callus Induction and Subculture of Lonicera japonica Thunb.[J].Northern Horticulture,2013,37(05):106-109.
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金银花愈伤组织的诱导及继代培养研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年05期 页码: 106-109 栏目: 出版日期: 2013-03-15
Title:
Study on Callus Induction and Subculture of Lonicera japonica Thunb.
文章编号:
1001-0009(2013)05-0106-04
作者:
单广福1; 2; 张晓丽1; 3; 李明军1; 3; 4; 陈明霞1; 3; 刘文英1; 芦婕1
1.河南师范大学 生命科学学院，河南 新乡 453007；
2.河南教育学院，河南 郑州 450046；
3.河南省高校道地中药材保育及利用工程技术研究中心，河南 新乡 453007；
4.新乡市中药材保育及利用工程技术研究中心，河南 新乡 453007
Author(s):
SHAN Guang-fu1; 2; ZHANG Xiao-li1; 3; LI Ming-jun1; 3; 4; CHEN Ming-xia1; 3; LIU Wen-ying1; LU Jie1
1.College of Life Science，Henan Normal University，Xinxiang，Henan 453007；
2.Henan Education Institute，Zhengzhou，Henan 450046；
3.Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs，University of Henan Province，Xinxiang，Henan 453007;
4.Engineering Technology Research Center of Nursing and Utilization of Chinese Crude Drugs，Xinxiang，Henan 453007
关键词:
金银花; 愈伤组织; 诱导; 继代
Keywords:
Lonicera japonica Thunb.; callus; induction; subculture
分类号:
S 567.7+9
文献标志码:
A
摘要:
以金银花优良品种“金丰一号”为试材，研究了不同外植体及不同浓度激素组合对金银花愈伤组织诱导和继代的影响。结果表明：愈伤组织诱导时，最佳外植体为花蕾下部，最佳培养基为MS＋NAA 2.0 mg/L＋6-BA 0.01 mg/L＋2,4-D 0.5 mg/L，诱导率可达100%，且愈伤组织生长较快，量多，质地疏松、湿润，颜色鲜亮；愈伤组织继代时，最佳培养基为MS＋6-BA 2.0 mg/L＋KT 0.75 mg/L＋NAA 0.25 mg/L，在此继代培养基上，经过3~5次继代后可以得到4种不同类型的愈伤组织。
Abstract:
Taking Lonicera japonica Thunb.‘Jinfeng No.1’ as material，the effect of different explants and different concentrations of plant growth regulators treatments on callus induction and subculture were studied.The results showed that the callus induction of the best explants was lower buds，and the best induction medium was MS＋NAA 2.0 mg/L＋6-BA 0.01 mg/L＋2,4-D 0.5 mg/L，the inducing ratio could reach 100%；the callus grew faster，plenty，moistly，loose，colors brightly.The best subculture medium for callus was MS＋6-BA 2 mg/L＋KT 0.75 mg/L＋NAA 0.25 mg/L，4 kinds of callus could be obtained after subcultured for 3~5 generations.

参考文献/References:

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备注/Memo

第一作者简介：单广福(1963-)，男，河南柘城人，本科，副教授，研究方向为植物学。E-mail:sgf-718 @163.com.
责任作者：李明军(1962-)，男，河南温县人，博士，教授，研究方向为药用植物生物技术。E-mail:limingjun2002@263.net.
基金项目：河南省教育厅科技攻关计划资助项目（2011B180034）；新乡市科技创新平台建设计划资助项目；新乡市科技攻关计划资助项目(08N041)。
收稿日期：2012-11-08

更新日期/Last Update: 2014-08-22