LI Jian-xin,GE Gui-min,PANG Shu-min,et al.Study on in vitro Culture and Plantlet Regeneration from Unpollinated Ovary of Cucumis sativus L.[J].Northern Horticulture,2012,36(23):131-134.
三个基因型黄瓜品种未授粉子房胚状体诱导及植株再生研究
- Title:
- Study on in vitro Culture and Plantlet Regeneration from Unpollinated Ovary of Cucumis sativus L.
- 文章编号:
- 1001-0009（2012）23-0131-04
- Keywords:
- Cucumis sativus L.; unpollinated overies; embryoid; regenerated plant
- 分类号:
- S 642.203.6
- 文献标志码:
- A
- 摘要:
- 以3个品种黄瓜的未授粉子房为外植体，进行胚状体诱导和植株再生研究。结果表明：3个品种都获得了再生植株。不同品种、不同热处理时间和不同TDZ浓度以及外植体的切割方式对胚状体诱导率都有影响。不同品种及不同6-BA和NAA浓度组合对植株的再生率有影响。热处理3 d，外植体为条状，TDZ浓度在0.06～0.08 mg/L时这3个品种的胚状体诱导率较高。NAA浓度为0.05 mg/L，6-BA浓度为0.4～0.6 mg/L时有利于胚状体分化植株。
- Abstract:
- Taking the unpollinated overies of them Henan Cucumis sativus L. as explant,the induction of embryoid and plant regeneration of three varieties of were studied.The results showed that regenerated plants were obtained in three varieties of cucumber.Different varieties，heat-treated time，concentration of TDZ and shape of explants affected the frequency of embryoid induction.Different varieties and concentration combinations of 6-BA and NAA affected the frequency of plant regeneration.Heat-treated for three days，sticks of explants and 0.06~0.08 mg/L of TDZ had high frequency of embryoid induction.0.05 mg/L of NAA and 0.4~0.6 mg/L of 6-BA could facilitate seedlings regeneration from embryoid.
参考文献/References:
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备注/Memo
第一作者简介：李建欣（1975-），女，硕士，助理研究员，现主要从事蔬菜生物技术育种研究工作。E-mail:lijianxin1007@163.com.