WANG Jing,LIU Li-gong,ZUO Li-juan,et al.Study on Tissue Culture and Rapid Propagation of Heuchera villosa[J].Northern Horticulture,2012,36(23):116-118.
柔毛矾根组培快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation of Heuchera villosa
- 文章编号:
- 1001-0009（2012）23-0116-03
- Keywords:
- Heuchera villosa; tissue culture; rapid propagation
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以柔毛矾根“香茅”和“饴糖”为试材，对其组培快繁技术进行系统研究。结果表明：以温室采集的芽作为外植体较适宜；初代培养最佳培养基配方为MS+6-BA 0.2 mg/L；MS+BA 0.2 mg/L+NAA 0.01 mg/L作为增殖培养基效果最好；生根阶段的最佳培养基为1/2MS+IBA 1.5 mg/L。用蛭石和珍珠岩1∶1的比例移栽苗时成活率可达95%以上。
- Abstract:
- The rapid micropropagation technology of Heuchera villosa ‘Citronelle’ and Heuchera villosa ‘Caramelr’ were investigated in the experiment.The results showed that the suitable explants was the bud in the greenhouse；the optimal medium for bud induction was MS+6-BA 0.2 mg/L；the best medium for successive transfer culture was MS+BA 0.2 mg/L+NAA 0.01 mg/L；and using 1/2MS+IBA 1.5 mg/L as rooting medium was better.Plantlets transplanted on media which contained vermiculite and perlite with 1∶1 had survival rate of more than 95%.
参考文献/References:
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备注/Memo
第一作者简介：王晶（1982-），女，硕士，讲师，研究方向为园林园艺植物。E-mail:wangjing0312@yahoo.com.cn.