LIU Kai-dong,YUAN Chang-chun,CHEN Yan,et al.Comparison on Effects of Different Methods on Extract Genomic DNA of Wild Litchi Leaves[J].Northern Horticulture,2012,36(23):105-109.
不同方法提取野生荔枝基因组DNA效果的比较
- Title:
- Comparison on Effects of Different Methods on Extract Genomic DNA of Wild Litchi Leaves
- 文章编号:
- 1001-0009（2012）23-0105-05
- Keywords:
- wild litchi; genomic DNA; SDS method; CTAB method
- 分类号:
- S 667.1
- 文献标志码:
- A
- 摘要:
- 以广东、广西地区的3个野生龙眼的幼嫩叶片为试材，采用SDS法、常规CTAB法和改良2×CTAB法提取荔枝叶片基因组DNA，比较研究从富含多酚、多糖和色素等次生代谢物质的野生荔枝叶片中获得高质量DNA的提取方法。结果表明：采用改良的2×CTAB法提取的荔枝叶片基因组DNA纯度最高、质量最佳，可直接用于荔枝叶绿体DNA trnL-F基因的PCR扩增分析，且扩增后条带清晰。表明改良的2×CTAB方法获得的DNA纯度和产率较高，符合进行野生分子标记的实验要求。
- Abstract:
- Experiment was conducted with three different leaves of wild litchi from Guangdong and Guangxi province.The genomic DNA extraction of three methods namely SDS，CTAB and modified 2×CTAB were compared.The aim was to obtain the high quality DNA extracted method from leaves of wild litchi which contained plenty of secondary substances such as polysaccharide，polyphenols，pigment and protein.The results showed that the modified 2×CTAB method could extract the genomic DNA from wild litchi leaves of the highest purity and best quality.It also could apply on the PCR amplificative analysis on the cpDNA trnL-F gene of wild litchi leaves with distinct strips after amplification.Therefore，the modified 2×CTAB method was the better method to extract the genomic DNA from wild litchi leaves.
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备注/Memo
第一作者简介：刘锴栋（1982-），男，广东电白人，硕士，讲师,研究方向为植物生物技术。E-mail:liukaidong2001@126.com.