LIU Xue-lian,QIN Jia-mei,SUN Jun-bo.Effect of Medium Components on Pollen Germination in vitro of Diervilla sessilifolia[J].Northern Horticulture,2012,36(24):129-131.
培养基组分对锦带花粉离体萌发的影响
- Title:
- Effect of Medium Components on Pollen Germination in vitro of Diervilla sessilifolia
- 文章编号:
- 1001-0009(2012)24-0129-03
- 分类号:
- S 685.99
- 文献标志码:
- A
- 摘要:
- 以锦带新鲜花粉为试材,采用正交设计L16(45),研究了蔗糖浓度、钙离子浓度、硼酸浓度对锦带花粉离体萌发的影响,以期筛选出锦带花粉离体萌发的最适宜培养基成分。结果表明:蔗糖浓度和钙离子浓度对花粉萌发和生长影响较大,硼酸的影响不如二者显著。锦带花粉离体萌发最适宜培养基成分为:蔗糖25%、硼酸0.016%、钙离子0.0050%。
- Abstract:
- Taking the brocade belt fresh pollen as material,using orthogonal design L16(45),the effect of sucrose density,the calcium ion density,the boric acid density on the brocade belt pollen exsomatize were studied,to screen brocade belt pollen exsomatize sprouts the culture medium ingredient which was suitable.The results showed that the sucrose density and the calcium ion density showed large effect on the pollen germination and the growth influence,the boric acid influence was inferior that the two were remarkable.The brocade belt pollen exsomatize sprouts that were most suitable the culture medium ingredient was:sucrose 25%,boric acid 0.016%,calcium ion 0.0050%.
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备注/Memo
第一作者简介:刘雪莲(1978-),女,硕士,讲师,现主要从事植物资源多样性保护与利用方面的研究工作。E-mail:liuxuelian1023@163.com.