[1]翁梁,温鲁.硒和钙对蛹虫草活性物质含量的影响[J].北方园艺,2012,36(22):159-161.
　WENG Liang,WEN Lu.Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances[J].Northern Horticulture,2012,36(22):159-161.

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硒和钙对蛹虫草活性物质含量的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年22期页码: 159-161 栏目: 出版日期: 2012-11-30

Title:: Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances

文章编号:: 1001-0009（2012）22-0159-03

作者:: 翁梁¹; 温鲁²; 1.江苏食品职业技术学院食品工程系，江苏淮安 223003；
2.淮阴师范学院资源微生物研究所，江苏淮安 223300

Author(s):: WENG Liang¹; WEN Lu²; 1.Department of Food Engineering，Jiangsu Food Science College，Huai’an，Jiangsu 223003;
2.Institute of Resource Microorganism，Huaiyin Normal University，Huai’an，Jiangsu 223300

关键词:: 亚硒酸钠; 氯化钙; 液体振荡培养; 活性物质

Keywords:: Cordyceps militaris; Na₂SeO₃; CaCl₂; liquid shake culture; active substances

分类号:: S 567.3+9

文献标志码:: A

摘要:: 向培养液中分别添加不同浓度的亚硒酸钠、氯化钙，用液体振荡培养法培养蛹虫草菌丝体，用HPLC法测定虫草素与腺苷含量，用苯酚-硫酸法测定多糖含量，用高碘酸钠比色法测定虫草酸含量。以研究2种微量元素对蛹虫草菌丝体中虫草素、腺苷、多糖和虫草酸含量的影响。结果表明：向培养液中添加适量的硒或钙，可显著提高蛹虫活性物质含量。添加15 mg/L亚硒酸钠，虫草素和虫草酸含量比对照组分别提高了29.0%和34.9%；添加16 mg/L氯化钙，虫草素含量比对照提高24.05%。

Abstract:: Adding different levels of Na₂SeO₃ and CaCl₂ to the culture medium，with the method of liquid shake culture，the effect of 2 trace elements on the content of cordycepin and adenosine，polysaccharide and cordyceps acid were studied，with HPLC to test the level of cordycepin and adenosine；with phenol-sulphate acid to test the level of polysaccharide；with sodium periodate colorimetric to test the level of cordyceps acid.The results showed that adding suitable selenium or calcium could obviously raised the content of Cordyceps militaris active substances.Adding 15 mg/L Na₂SeO₃ to the culture medium and the level of cordycepin and cordyceps acid had raised 29.0% and 34.9% respectively.Adding 16 mg/L CaCl₂ and the level of cordycepin had raised 24.05%.

参考文献/References:

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[2]韦会平,肖波,胡开治.蛹虫草药用价值考[J].中药材,2004,27(3):215-217.

[3]林群英,宋斌,李泰辉.蛹虫草研究进展[J].微生物学通报,2006,33(4):154-157.

[4]王志高,温鲁,袁小传,等.加硒对蛹虫草主要活性成分含量的影响[J].安徽农业科学,2007,35（29）:93-94.

[5]夏敏,温鲁.微波法提取虫草素研究[J].食品科学,2006,27(10):248-251.

[6]翁梁，温鲁.TLCS与HPLC对比测定虫草中虫草素和腺苷含量[J].江苏农业学报,2008,24（4）:539-540.

[7]翁梁,温鲁,杨芳,等.不同提取方法对蛹虫草活性多糖抗氧化性影响[J].食品科技,2008,33(11):180-182.

[8]温鲁,翁梁,朱明伟,等.不同林区蛹虫草活性成分含量的比较[J].林业科学,2008,44(8):149-151.

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备注/Memo

第一作者简介：翁梁（1982-），男，硕士，助教，研究方向为生物活性物质与功能食品。E-mail:wjwengliang@126.com.

更新日期/Last Update: 2014-08-31