LI Jin-ying,ZHAO Chun-li,ZHANG Zhi-dong.Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.‘Hanfeng’[J].Northern Horticulture,2012,36(22):119-121.
黑穗醋栗‘寒丰’离体快繁影响因素研究
- Title:
- Study on the Factors Influencing Rapid Propagation in vitro of Ribes nigrum L.‘Hanfeng’
- 文章编号:
- 1001-0009（2012）22-0119-03
- Keywords:
- Hanfeng’; rapid propagation in vitro
- 分类号:
- S 663.903.6
- 文献标志码:
- A
- 摘要:
- 以黑穗醋栗‘寒丰’植株不同组织部位为外植体，研究分析了离体快繁过程中的一些影响因素。结果表明：‘寒丰’茎尖消毒时间以0.1%升汞（HgCl2）消毒7 min，带腋芽的茎段9 min为宜，嫩茎以手摇消毒效果较好，其它辅助材料以0.1%升汞+2滴吐温消毒8 min效果较佳；‘寒丰’组培苗在MS+BA 1.0 mg/L+IBA 0.1 mg/L+GA3 1.0~2.0 mg/L培养基上能分化出大量丛生芽，且长势较好。
- Abstract:
- Taking different part of Ribes nigrum L.‘Hanfeng’ as materials,the factors that influencing culture in vitro of ‘Hanfeng’ were studied by in vitro culture of different parts of organs，including stem tip and stem with auxiliary bud.The results showed that the optimal sterilization time of stem tip was 7 minutes with 0.1% HgCl2 and 9 minutes of auxiliary bud,tender stem with hand shelce had the better sterilize effect,0.1 HgCl2 plus two drops of Twain 8 min had betler effect.The cluster buds could be induced in MS+BA 1.0 mg/L+IBA 0.1 mg/L+GA3 1.0~2.0 mg/L，and the plants grew well.
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备注/Memo
第一作者简介：李金英（1978-），女，吉林长春人，在读博士，实验师，研究方向为果树生物技术。E-mail:li_jy78@163.com.