DAI Ping,ZHANG Chun-xia,ZHANG Zhi,et al.Tissue Culture and Rapid Propagation of Lycoris chinensis[J].Northern Horticulture,2012,36(22):95-98.
中国石蒜的组织培养和快速繁殖
- Title:
- Tissue Culture and Rapid Propagation of Lycoris chinensis
- 文章编号:
- 1001-0009（2012）22-0095-04
- Keywords:
- Lycoris chinensis Traub; tissue culture; rapid propagation
- 分类号:
- S 682.2+9
- 文献标志码:
- A
- 摘要:
- 以中国石蒜的种胚为外植体，进行了组织培养与快速繁殖技术的研究。结果表明：种子表面消毒以75%酒精预处理30 s，再用0.1% HgCl2 浸泡15 min效果最好；愈伤组织诱导的最佳培养基为MS+2,4-D 2.0 mg/L；芽分化诱导最佳增殖培养基为MS+6-BA 5.0 mg/L+NAA 0.5 mg/L；小鳞茎生长最佳培养基为MS+6-BA 3.0 mg/L+NAA 3.0 mg/L；在理想的根分化培养基MS+NAA 1.0 mg/L上，生根率达到81%。练苗后，移入营养土与珍珠岩（1∶1）的基质中，移栽成活率达到78%。
- Abstract:
- Seed mbryoes of Lycoris chinensis were used as explant in the study of tissue culture and rapid propagation.The results showed that the most suitable procedure for sterilization was to soak seeds into 0.1% HgCl2 solution for 15 min after retreatment with 75% alcohol for 15 s.The results indicated that the best induced medium for callus induction was MS+2,4-D 2.0 mg/L.The best medium for bud differentiation was MS+6-BA 5.0 mg/L+NAA 0.5 mg/L.The best medium for little bulbs growth was MS+6-BA 3.0 mg/L+NAA 3.0 mg/L.The most optimum rooting medium was MS+NAA 1.0 mg/L，and the rooting rate was 81%.The rooting seedlings were effectively transplanted into base materials which contained nutritious soil and perlite (1∶1).The survival rate was up to 75%.
参考文献/References:
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备注/Memo
第一作者简介：戴萍（1987-），女，在读硕士，现主要从事石蒜生长发育及繁殖方面的研究工作。