HE Ying-ying,CHEN Wei-min,ZHAO Yong-chang,et al.Genetic Diversity Analysis of Two Agrocybe Species in Yunnan by AFLP[J].Northern Horticulture,2012,36(21):85-88.
云南田头菇属两个物种遗传多样性的AFLP分析
- Title:
- Genetic Diversity Analysis of Two Agrocybe Species in Yunnan by AFLP
- 文章编号:
- 1001-0009（2012）21-0085-04
- 分类号:
- S 646.1+9
- 文献标志码:
- A
- 摘要:
- 利用扩增片段长度多态性（AFLP）技术分析了田头菇属的茶树菇（Agrocybe aegerita）及杨柳田头菇（Agrocybe.salicacola）基因组DNA的多态性，使用11对引物组合建立了18个供试菌株的指纹图谱。结果表明：通过聚类分析，18个供试菌株聚为两大类，彼此关系得到很好的分辨。AFLP技术可用于茶树菇和杨柳田头菇2个形态上非常相似物种的DNA分子指纹图谱构建。
- Abstract:
- AFLP technique was used to analyze the genomic DNA polymorphism of Agrocybe aegerita and A.salicacola collected from Yunnan.The DNA fingerprint of the eighteen strains in Agrocybe was generated by eleven primer combinations.Eighteen strains of Agrocybe were clustered into two big groups，which phylogenic relationship was distinguished clearly.The results indicated that AFLP technique could be used to construct the DNA molecular fingerprint of A.aegerita and A.salicacola, both of which were very similar in morphology.
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备注/Memo
第一作者简介：何莹莹（1984-），女，硕士，研究方向为大型真菌分子生物学。E-mail:hyy148427952@163.com.