TAN Chao,ZHANG Jian-xia,WANG Yue-jin.Study on Agrobacterium-mediated Transformation of Vitis vinifera (L.) and Plant Regeneration[J].Northern Horticulture,2012,36(19):113-117.
欧洲葡萄体胚诱导及农杆菌介导的遗传转化研究
- Title:
- Study on Agrobacterium-mediated Transformation of Vitis vinifera (L.) and Plant Regeneration
- 文章编号:
- 1001-0009（2012）19-0113-05
- 分类号:
- S 663.103.6
- 文献标志码:
- A
- 摘要:
- 以欧洲葡萄（Vitis vinifera L.）品种“无核白”（‘Thompson Seedless’）、“佳利酿”（‘Carignane’）、“红地球”（‘Red Globe’）的雄蕊、雌蕊和花蕾为外植体，对花器官体细胞胚诱导及农杆菌介导的转芪合成酶基因进行研究。结果表明：所采用的5种胚性愈伤组织诱导培养基中，MC培养基较适于雄蕊胚性愈伤组织的诱导，“无核白”、“佳利酿”和“红地球”雄蕊的胚性愈伤组织诱导率分别为1.7%、0.3%和11.8%；PIV培养基可诱导“无核白”和“佳利酿”雌蕊胚性愈伤组织形成，诱导率分别为4.0%和3.2%，而“红地球”雌蕊在MC培养基上诱导率最高，达5.6%；仅“红地球”的花蕾在MS1培养基上可诱导产生胚性愈伤组织，诱导率为0.5%。改良X6液体培养基较有利于次生胚的诱导；B培养基有利于体细胞胚萌发成苗。转基因材料经抗性筛选和植株再生，获得了2株“无核白”抗性苗，经PCR和PCR-Southern检测表明，目的基因被整合到转基因植株中。
- Abstract:
- Stamen，pistils and flower buds from Vitis vinifera cvs.‘Thompson Seedless’，‘Carignane’ and ‘Red Globe’ were used as explants to investigate their efficience of embryonic callus induction and Agrobacterium-mediated stilbene synthase gene transformation.The results indicated that MC medium was optimum for embryonic callus induction from stamens among 5 medium for embryonic callus induction.The induction rate in ‘Thompson Seedless’，‘Carignane’ and ‘Red Globe’ were 1.7% 0.3% and 11.8%，respectively.Embryonic callus from ‘Thompson Seedless’ and ‘Carignane’ could be induced in PIV medium with 4.0% and 3.2% induction rates.However，the highest induction rate occurred in embryonic callus from flower buds of ‘Red Globe’ in MC medium with 5.6% induction rate.Only flower buds from Redglobe could be induced in MS1 medium with 0.5% induction rate.A modified X6 liquid medium was suitable for secondary embryogenesis，and medium B favored germination and seedling of somatic embryos.Transformed plants were selected through resistance screening and 2 resistent plants of ‘Thompson Seedless’ were obtained.Results from PCR and PCR-Southern indicated that stilbene synthase gene were integrated into the genome of ‘Thompson Seedless’.
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备注/Memo
第一作者简介：谭超（1985-），男，在读硕士，研究方向为葡萄遗传育种。E-mail：tanchaos@163.com.