QIAO Yong-xu,ZHANG Yong-ping,ZHANG Hong-xin,et al.Establishment of Regeneration System of Gerbera[J].Northern Horticulture,2012,36(18):133-134.
非洲菊再生体系的建立
- Title:
- Establishment of Regeneration System of Gerbera
- 文章编号:
- 1001-0009(2012)18-0130-03
- Keywords:
- gerbera; receptacle; callus; buds occurring
- 分类号:
- S 681.9
- 文献标志码:
- A
- 摘要:
- 以非洲菊幼嫩花托为外植体,以MS为基础培养基,研究了添加6-BA和NAA的培养基对愈伤组织诱导、丛生芽发生和丛生芽生根的影响。结果表明:花托的大小影响到愈伤组织的诱导和褐化,0.8 cm的花托愈伤组织的诱导率高且褐化率低;愈伤组织诱导和芽分化的适宜培养基为MS+6-BA 10 mg/L+NAA 0.2 mg/L,丛生芽增值的适宜培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,生根培养基为1/2 MS+IAA 0.2 mg/L。
- Abstract:
- ‘Gerbera’ receptacle was used as explant,the effect of callus, buds and roots induction at different concentration in 6-BA and NAA were studied. The results showed that 0.8 cm receptacle callus induction rate was high but browning was low. The best callus and buds induction mediums was MS+6-BA 10 mg/L+NAA 0.2 mg/L. The best buds breeding mediums was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L. Root occurred mediums was 1/2 MS+IAA 0.2 mg/L.
参考文献/References:
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备注/Memo
第一作者简介:乔永旭(1978-),男,硕士,副教授,研究方向为植物细胞工程和植物资源开发与利用。E-mail:qiaoyx123@163.com.