LIU Jin-feng,LI Yong-hua,WU Hui-ping.Study on Tissue Culture and Rapid Propagation Technology of Ziziphus jujuba Mill[J].Northern Horticulture,2012,36(16):98-100.
“中宁小枣”组培快繁技术研究
- Title:
- Study on Tissue Culture and Rapid Propagation Technology of Ziziphus jujuba Mill
- 文章编号:
- 1001-0009(2012)16-0098-03
- Keywords:
- Ziziphus jujuba Mill; adventitious buds; tissue culture
- 分类号:
- S 665.103.6
- 文献标志码:
- B
- 摘要:
- 以宁夏“中宁小枣”种仁为外植体，进行了愈伤组织、不定芽诱导、分化、生根试验研究。结果表明：MS+6-BA 1.0 mg/L+NAA 1.0 mg/L为最适合种子萌芽诱导的培养基；MS+KT 2.0 mg/L+IBA 1.0 mg/L+GA31.0 mg/L为最佳试管苗分化的培养基；IBA和NAA能明显促进难繁植物生根反应，1/2MS+IBA 0.8 mg/L+NAA 0.2 mg/L为最适合试管苗生根培养基。
- Abstract:
- Taking Ziziphus jujuba acinus as explants,the induction of the buds,differentiation,taking roots and transplants were studied.The results showed that MS+6-BA 1.0 mg/L+NAA 1.0 mg/L was the most suitable medium for the induction of adventitious buds.MS+KT 2.0 mg/L+IBA 1.0 mg/L+GA3 1.0 mg/L was the most suitable medium for differentiation.IBA and NAA promoted root formation,1/2MS+IBA 0.8 mg/L+NAA 0.2 mg/L was the most suitable condition for inducing roots.
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备注/Memo
第一作者简介：柳金凤（1983-），女，硕士,助理研究员,现主要从事难繁植物组培快繁及植物基因工程技术研究工作。E-mail:liujinfeng_317@163.com.