SUN Rui,SHAO Hong,LIU Juan,et al.Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase[J].Northern Horticulture,2012,36(16):88-89.
北马兜铃营养生长期过氧化物酶同工酶分析
- Title:
- Study on POD Isoenzymes of Aristolochia contorta Bunge at Vegetative Growth Phase
- 文章编号:
- 1001-0009(2012)16-0088-02
- Keywords:
- Aristolochia contorta Bunge; vegetative growth phase; POD; isoenzyme
- 分类号:
- S 567.23；Q 946.5
- 文献标志码:
- B
- 摘要:
- 用聚丙烯酰胺垂直板凝胶电泳技术对北马兜铃营养生长期根、幼茎、老茎、叶柄、未展开幼叶、展开幼叶和成熟叶的过氧化物酶（POD）同工酶进行了分析。结果表明：北马兜铃营养生长期7个组织器官共有10条POD酶带，其POD同工酶具有组织特异性，其中未展开幼叶的POD同工酶酶带丰富、活性较强、含量较高，是POD同工酶分析最好的材料，而成熟叶片由于POD酶种类太少，最不适合作POD同工酶分析。
- Abstract:
- The POD enzymes from root，tender stem，old stem，petiole，unexpanded tender leaf，expanded tender leaf and mature leaf of Aristolochia contorta Bunge at vegetative growth phase were analyzed by vertical polyacrylamide gel electrophoresis (PAGE).The results showed that 10 bands of POD isoenzymes from 7 different tissues and organs of Aristolochia contorta Bunge at vegetative growth phase were expressed and POD isoenzymes had a tissue specificity.Unexpanded tender leaf which had abundant enzyme bands，stronger activity and higher content were the good materials for analysis on POD isoenzyme.There were the fewest enzyme bands in mature leaf，so it was not fit for analysis on POD isoenzyme.
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备注/Memo
第一作者简介：孙睿(1978-)，男，在读硕士，讲师，研究方向为植物遗传育种学与植物生物技术。E-mail:sr_sh@163.com.