JIANGLan-jun,GONGZhen-hui,ZHAOQian.StudyonRejuvenation,PreservationMethodsandGrowthCharacteristicsof ThreePhysiologicalRacesofPhytophthoracapsici[J].Northern Horticulture,2012,36(12):161-164.
辣椒疫霉菌复壮与保存及生长特性研究
- Title:
- StudyonRejuvenation,PreservationMethodsandGrowthCharacteristicsof ThreePhysiologicalRacesofPhytophthoracapsici
- 文章编号:
- 1001-0009（2012）12-0161-04
- Keywords:
- Phytophthoracapsici; physiologicalraces; backinoculation; rejuvenation; preservation; growthrate
- 分类号:
- S436.418.1+9
- 文献标志码:
- A
- 摘要:
- 研究了辣椒疫霉菌3个生理小种的孢子囊诱导、回接复壮、短期固体培养基保存方法和复壮、培养基厚度、菌龄、生理小种对菌落生长及其形态的影响。结果表明：打菌饼诱孢较整皿光照诱孢速度快、产孢量大；发病辣椒茎段较发病叶片回接效率高，达到50%；疫霉菌在16℃下固体培养基上保存效果最好，保存时间为3~4个月；辣椒疫霉菌复壮前后Ph1、Ph3生理小种菌丝生长速率无显著差异，Ph2有显著差异，复壮后3个生理小种菌丝都变致密，致病性增强；培养基厚度可影响菌丝生长速率，10~15mL体积的培养基较适合疫霉菌的培养；疫霉菌菌丝的菌龄对菌丝生长速率无影响；Ph1、Ph2、Ph3随着致病性的增强菌丝生长速率下降。
- Abstract:
- Researchesonmethodsofsporangiuminducing,rejuvenation,preservationandgrowthcharacteristicsofPhytophthoracapsiciwerecarriedout.Theresultsshowedthatthesporangiumonmycelialplugsweremoreandappearedearlierthanthemyceliumpickingoutfromthewholecolonyunderlight;Theefficiencyofstemsegments’backinoculationwashigherthanthatoftheleafsegments;ThepreservationeffectonPSAmediumunder16℃wasbestandpreservationtimewasthreetofourmonths;GrowthrateofPh1andPh3strainsexceptPh2hadnosignificantdifferencespre-andpost-rejuvenationbutthecolonyofthreephysiologicalracesbecamecompactandpathogenicityenhancedafterrejuvenation;Thethicknessofmediumhadeffectonthegrowthofcolonyandthebestvolumewas10~15mL;Thecellageofmyceliumhadnoeffectonthegrowthofcolony;Thegrowthrateofthreephysiologicalracesslowedastheirpathogenicityenhanced.
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备注/Memo
第一作者简介：蒋兰君(1986-)，女，山东潍坊人，在读硕士，研究方向为蔬菜生物技术与遗传育种。E-mail：jianglanjunmo@163.com。
责任作者：巩振辉（1957-），男，陕西礼泉人，博士，教授，博士生导师，现主要从事蔬菜生物技术与遗传育种研究工作。E-mail：gzhh168@yahoo.com.cn。
基金项目：国家自然科学基金资助项目(30771467)；“十二五”农村领域国家科技计划资助项目（2011BAD12B00）；教育部高校博士点基金资助项目(200807120007)。
收稿日期：2012-03-19