XIUJing-run,PIAOXuan-chun,YAORui,et al.EffectofSeveralExternalFactorsonProliferationandGrowthofDendrobiumcasiflake[J].Northern Horticulture,2012,36(12):143-145.
培养基种类、BA浓度和蔗糖浓度对春石斛组培苗增殖的影响
- Title:
- EffectofSeveralExternalFactorsonProliferationandGrowthofDendrobiumcasiflake
- 文章编号:
- 1001-0009(2012)12-0143-03
- Keywords:
- Dendrobiumcasiflake; proliferationandgrowth; mediumtypes; BAconcentration; sucroseconcentration
- 分类号:
- S681.903.6
- 文献标志码:
- A
- 摘要:
- 以春石斛(Dendrobiumcasiflake)的组培苗为试材,研究培养基的种类、BA浓度、蔗糖浓度等因素对不定芽增殖生长的影响,为春石斛种苗的规模化生产提供理论依据。结果表明:在MS培养基中不定芽分化及生长能力明显好于White、VW、SH、B5及KC培养基,培养30d后每个外植体平均分化出3.7个健壮的苗;在MS培养基中加入BA3.0mg/L以及蔗糖30g/L时,春石斛不定芽增殖数多,且生长良好。
- Abstract:
- WithadventitiousshootsproliferatedthroughtissuecultureofD.casiflakeasexperimentalmaterial,theeffectofseveralfactorssuchasthemediumtypes,theconcentrationsofBA,theconcentrationsofsucroseetcontheproliferationandgrowthofD.casiflakewerestudied.ToprovidethetheoreticalbasisforscaleproductionofD.casiflakeseedling.TheresultsshowedthattheeffectofMSmediumontheproliferationandgrowthofD.casiflakewasbatterthanotherkindsofmedium,andshouldhelpimprovethedifferentiationofadventitiousshoots.Ithadobviouspromotingeffectonthedifferentiationofadventitiousshoots,freshweightanddryweightwhenBAat3.0mg/Landsucroseat30g/LwereaddedintoMSmediumwhichwereperfectfortheproliferationandgrowthofD.casiflake.
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相似文献/References:
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备注/Memo
第一作者简介:修景润(1981-),男,硕士,现主要从事长白山经济植物栽培育种研究工作。E-mail:huijuanli2006@163.com。
责任作者:廉美兰(1963-),女,博士,教授,现主要从事植物组织培养和生物反应器的研究工作。
基金项目:国家自然科学基金资助项目(31160179)。
收稿日期:2012-03-26