XUE Qi-qin,LI Mei-qin,PEI Hua-li,et al.Study on Callus Induction and Efficient Regeneration System in Different Varieties of Anthurium andraeanum[J].Northern Horticulture,2012,36(10):130-132.
不同红掌品种愈伤组织诱导及高效再生体系的建立
- Title:
- Study on Callus Induction and Efficient Regeneration System in Different Varieties of Anthurium andraeanum
- 文章编号:
- 1001-0009（2012）10-0130-03
- Keywords:
- Anthurium andraeanum; cllus; induction; plant regeneration
- 分类号:
- S 682.1+4
- 文献标志码:
- A
- 摘要:
- 以“火焰”、“阿拉巴马”和“粉冠军” 3个红掌盆花品种的叶柄、叶片为外植体，对红掌愈伤组织诱导及植株再生进行了研究，建立了红掌的高效再生体系。结果表明：红掌的叶柄较叶片更适宜愈伤组织的诱导,叶柄为外植体诱导愈伤组织的最适培养基：1/2MS+1.5 mg/mL 6-BA+0.6 mg/mL 2,4-D，诱导率为81.7%；以叶片为外植体诱导愈伤组织的最适宜培养基为：1/2MS+1.0 mg/mL 6-BA +0.8 mg/mL 2,4-D，诱导率为65.0%；“火焰”的愈伤组织诱导率最高，其次为“阿拉巴马”，“粉冠军”的愈伤组织诱导率最低。以叶柄和叶片作为外植体诱导产生的愈伤组织最适宜分化培养基为：1/2MS+1.0 mg/mL 6-BA +0.2 mg/mL NAA，分化率分别达到88.3%和73.3%；“火焰”的芽分化率仍最高，“阿拉巴马”次之，“粉冠军”的芽分化率最低。幼苗生根的最适宜培养基为:1/2MS+0.5 mg/mL NAA。草炭土+珍珠岩（1∶1）混合基质是理想的移栽基质,移栽成活率为95%。
- Abstract:
- The petioles and blade of three Anthurium andraeanum varieties of ‘Fire’,‘Alabama’,‘Pink Champion’ were used as explants，the callus induction and plantlet regeneration of Anthurium andraeanum were studied,and its highly efficient regeneration system were established.The results showed that petioles were suitable explants in callus induction.The optimum medium for callus induction used petioles as explants was 1/2MS medium supplemented with 1.5 mg/mL 6-BA and 0.6 mg/mL 2,4-D,induction rate was 81.7%；The optimum medium for callus induction used blade as explants was 1/2MS medium supplemented with 1.0 mg/ mL 6-BA and 0.8 mg/mL 2,4-D,induction rate was 65.0%；The rates of callus induction and bud differentiation of Anthurium andraeanum ‘Fire’ were highest.And the rates of callus induction and bud differentiation of A.andraeanum “Alabama” were higher than those of A.andraeanum‘Pink champion’.The optimum medium for bud differentiation used petioles and blade as explants were 1/2MS medium supplemented with 1.5 mg/mL 6-BA and 0.6 mg/mL 2,4-D，induction rate were 88.3% and 73.3% respectively；The most suitable rooting medium was 1/2MS medium supplemented with 0.5 mg/mL NAA.Turfy soil and perlite(1∶1) was useful for transplantation，and the survival rate was 95%.
参考文献/References:
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备注/Memo
第一作者简介：薛其勤（1981-），男，山东莱芜人，硕士，讲师，现主要从事植物生物技术和宏观农业等研究工作。E-mail: xueqiqin@163.com。
责任作者：李美芹（1968-），女，博士，副教授，现主要从事生物学研究工作。E-mail:mqli901@126.com。
基金项目：山东省高等学校科技计划资助项目（J10LC78）；山东省优秀中青年科学家科研奖励基金资助项目（2009BSA07005）。
收稿日期：2012-02-27