WANG Zhong-wu,JIAN De-feng.Study on the Shoot-tip Culture of Hydrangea macrophylla in vitro[J].Northern Horticulture,2012,36(09):129-130.
八仙花茎尖离体培养技术研究
- Title:
- Study on the Shoot-tip Culture of Hydrangea macrophylla in vitro
- 文章编号:
- 1001-0009（2012）09-0129-02
- Keywords:
- Hydrangea macrophylla; culture in vitro; medium
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 以八仙花带芽枝条为试材，采用茎尖离体培养技术对八仙花进行快繁研究。结果表明:把经过消毒灭菌的茎尖切割后转入芽诱导培养基MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+GA3 1.0 mg/L,萌发率达到85%;继代培养基采用MS+6-BA 0.5 mg/L+NAA 0.04 mg/L+腺嘌呤5.0 mg/L，会具有较高的生长系数;生根培养基采用1/2MS+IBA 0.8 mg/L+活性炭3 g/L,能够完全生根成苗。
- Abstract:
- Hydrangea macrophylla was chosen as test material,the rapid propagation technique of Hydrangea macrophylla by shoot-tip culture in vitro were researched.The results showed that the shoot-tip after disinfected were seeded into the bud induction medium of MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+GA31.0 mg/L，and the germination rate reached 85%；Using MS+6-BA 0.5 mg/L+NAA 0.04 mg/L+adenine 5.0 mg/L as the continue medium could had higher growth factor；using 1/2MS+IBA 0.8 mg/L+ Activated carbon 3 g/L as rooting medium could fully take root into plants .
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备注/Memo
第一作者简介：王忠武（1969-），男，硕士，副教授，现主要从事植物科学教学和科研工作。E-mail：82642444@qq.com。
收稿日期：2012-02-07