WANG Zhen-hua,PANG Ji-liang,CHEN Yi-hong,et al.Study on Regenerating Shoots from Cotyledon Segment Cultures in Osmanthus fragrans[J].Northern Horticulture,2012,36(09):126-128.
桂花子叶切块培养再生苗的研究
- Title:
- Study on Regenerating Shoots from Cotyledon Segment Cultures in Osmanthus fragrans
- 文章编号:
- 1001-0009（2012）09-0126-03
- Keywords:
- Osmanthus fragrans; cotyledon segments; in vitro culture; adventitious bud differentiation; thidiazuron
- 分类号:
- S 685.13
- 文献标志码:
- A
- 摘要:
- 以桂花品种大花金桂(Osmanthus fragrans ‘Dahuajingui’)的子叶切块为外植体，研究了不同激素水平对桂花子叶切块愈伤组织诱导、芽分化和试管苗生根的影响。结果表明：诱导出的愈伤组织在分化培养基（1/2MS+0.5 mg/L TDZ+0.1 mg/L NAA）上继代培养9次后分化不定芽，不定芽分化率为22.86%，平均再生苗数为3.22，继代培养10次后，不定芽分化率高达36%。进行生根试验，确定桂花最佳生根培养基为1/2MS+0.5 mg/L IBA+2.0 mg/L NAA，生根率达到70%。
- Abstract:
- Cotyledon segments of Osmanthus fragrans ‘Dahuajingui’ were used as explant，the effect of hormone levels on the callus induced of cotyledons sliced of osmanthus,shoot differentiation and tube seedlings rooting were studied.The results showed that adventitious buds were differentiated from cotyledon segment calli after subculturing 9 times on differentiated medium （1/2MS+0.5 mg/L TDZ+0.1 mg/L NAA）Differentiation rate of the adventitious bud was 22.86%,and average number of.regenerating shoots was 3.22.After cotyledon segment calli were subcultured 10 times on same differentiated medium，frequency of the adventitious bud differentiation was up to 36%.The optimum rooting medium was 1/2MS+0.5 mg/L IBA+2.0 mg/L NAA,and the rooting rate could reach 70%.
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备注/Memo
第一作者简介：王珍华（1985-），女，在读硕士，现从事植物发育研究工作。E-mail:wangzhenhua_2005@163.com。
责任作者：庞基良（1963-），男，硕士，教授，硕士生导师，现从事植物发育生物学研究工作。 E-mail:pangrenshuiliang@yahoo.com.cn。
基金项目：国家自然科学基金资助项目（31071818）；浙江省重点科技创新团队资助项目（2010R50039）。
收稿日期：2012-01-05