CAO Dong-xu,ZHOU Hai-feng,YU Sheng-cheng,et al.Study on Heat-treated Stem Tip Culture of Kyoho Grape[J].Northern Horticulture,2012,36(09):116-118.
“巨峰”葡萄热处理茎尖培养技术研究
- Title:
- Study on Heat-treated Stem Tip Culture of Kyoho Grape
- 文章编号:
- 1001-0009（2012）09-0116-03
- Keywords:
- ‘Kyoho’ grape; stem tip; tissue culture
- 分类号:
- S 663.103.6
- 文献标志码:
- A
- 摘要:
- 以“巨峰”葡萄为试材,经热处理后取其茎尖进行离体培养，通过调整激素的种类和浓度，筛选出了适宜“巨峰”葡萄茎尖分化和生根的培养基；通过对不同移栽基质的研究，筛选出了移栽成活率较高的基质配方。结果表明：38℃热处理时葡萄苗发芽率最高，可达86.67%；以GS为基本培养基，附加BA 0.5 mg/L与NAA 0.1 mg/L，最适宜于“巨峰”葡萄茎尖分化成苗，分化率可达70.00%；生根培养基以GS+IBA 0.06 mg/L的生根率最高。适宜“巨峰”葡萄茎尖组培苗移栽的基质为河沙1份+水苔1份。
- Abstract:
- Kyoho grape was used as test material,stem tip was cultured after heat treatment,optimum medium formulas for multiple shoot differentiation from stem tip and for rooting were separately selected by adjusting the category and concentration of hormones,and optimum substrate for transplanting was also gained.The results indicated that the highest germination rate 86.67%was obtained by heat treatment at 38℃;GS+BA 0.5 mg/L+ NAA 0.1 mg/L was suitable for multiple shoot differentiation from stem tip, with the differentiation rate 70.00%, and GS+IBA 0.06 mg/L was optimum formula for rooting.The equivalence mixture of riversand and water moor was optimum substrate for transplanting.
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备注/Memo
第一作者简介：曹冬煦（1979-）,男,硕士,实验师,研究方向为遗传育种及组织培养。E-mail：13322475522@189.cn。
责任作者：年玉欣（1978-）,女,硕士,讲师,研究方向为植物遗传育种与繁育。E-mail：nianyuxin3@126.com。
收稿日期：2011-02-06