ZHANG Hui-jie,WANG Li-ying,YUAN Ting,et al.Research on Factory Breeding Tissue Culture Technology of Lonicera× tellmanniana Spaeth[J].Northern Horticulture,2012,36(08):131-133.
台尔曼忍冬组织培养技术工厂化繁育研究
- Title:
- Research on Factory Breeding Tissue Culture Technology of Lonicera× tellmanniana Spaeth
- 文章编号:
- 1001-0009（2012）08-0131-03
- Keywords:
- Lonicera×tellmanniana Spaeth; coefficient of differentiation; survival rate of transplanting
- 分类号:
- S 687.3
- 文献标志码:
- A
- 摘要:
- 以台尔曼忍冬为试材进行织培养工厂化繁育。结果表明：台尔曼忍冬适宜的分化培养基配方为MS+0.6 mg/L 6-BA+0.1 mg/L NAA+100 mg/L水解乳蛋白,分化系数为3.2；筛选出适宜的生根培养基配方为1/2MS+0.6 mg/L 6-BA+0.1 mg/L NAA,生根率92%;练苗5~7 d后，在草炭∶蛭石∶珍珠岩=2∶1∶1的基质中移栽,成活率90%;大田定植成活率为95%。该研究建立了台尔曼忍冬组织培养技术工厂化繁育体系,达到了高效快速,且再生繁育体系稳定性好，可为今后的台尔曼忍冬组织培养繁殖技术提供参考。
- Abstract:
- With Lonicera×tellmanniana Spaeth as test material,factory breeding tissue culture technology were studied.The results showed that appropriate differentiation medium of Lonicera×tellmanniana Spaeth was MS+0.6 mg/L 6-BA+0.1 mg/L NAA+100 mg/L hydrolyzed milk protein the coefficient of differentiation was 3.2；the best formula for the rooting medium was 1/2MS+0.6 mg/L 6-BA+0.1 mg/L NAA，the rate of rooting was 92%；after acclimatized for 5~7 d,the rate of survival was 90%，when the regeneration plantlets were transplanted to substate containing peat∶vermiculite∶perlite (2∶1∶1).The industrialization breed rate by tissue culture technology of Lonicera×tellmanniana Spaeth established through this study was rapid，the stability of regeneration system was well,could provide reference for the future tissue culture propagation technology of the Lonicera×tellmanniana Spaeth.
参考文献/References:
[1]谷淑芬,李长海,张旭东.台尔曼忍冬引种与繁殖技术[J].林业科技,1999,24(1):5-6.
相似文献/References:
[1]张慧洁,王立英,沈 军,等.台尔曼忍冬嫩枝扦插繁育技术研究[J].北方园艺,2014,38(08):48.
ZHANG Hui-jie,WANG Li-ying,SHEN Jun,et al.Research on Softwood Cutting Breeding Technology of Lonicera×tellmanniana Spaeth[J].Northern Horticulture,2014,38(08):48.
[2]刘安成,李艳,王庆,等.西安地区台尔曼忍冬栽培管理技术[J].北方园艺,2024,(11):152.[doi:10.11937/bfyy.20233685]
LIU Ancheng,LI Yan,WANG Qing,et al.Cultivation and Management Techniques of Lonicera×tellmanniana in Xi′an Area[J].Northern Horticulture,2024,(08):152.[doi:10.11937/bfyy.20233685]
备注/Memo
第一作者简介：张慧洁（1984-），女，河南太康人，本科，研究方向为植物组培技术。