LI Fang,REN Xue-qin,SUN Yang-wu,et al.In vitro Rapid Propagation of Weigela florida cv.Red Prince Stem-segment[J].Northern Horticulture,2012,36(08):115-117.
红王子锦带茎段组培快繁体系的建立
- Title:
- In vitro Rapid Propagation of Weigela florida cv.Red Prince Stem-segment
- 文章编号:
- 1001-0009（2012）08-0115-03
- 分类号:
- S 685.99
- 文献标志码:
- A
- 摘要:
- 以当年生红王子锦（Weigela florida cv.Red Prince）幼嫩带有腋芽的茎段为外植体，MS为基本培养基，通过不同的外植体消毒方式筛选出消毒的最佳方式和最佳时间，通过对植物生长激素的种类和浓度的调整，筛选出红王子锦带的最佳扩繁培养基和最佳生根培养基。结果表明：使用0.1%的HgCl2消毒7 min效果优于其它时间和NaClO的消毒效果，最佳的继代增值培养基为MS+BA 2.0 mg/L+NAA 0.1 mg/L+KT 2.0 mg/L，其丛芽诱导率为4.2。对扩繁苗进行生根培养，最佳生根培养基为1/2MS+IBA 0.6 mg/L，移栽至营养土中栽培，成活率为100%。
- Abstract:
- The stem-segments with axillary buds of Weigela florida cv. Red Prince were used as the explant.MS was used as basic medium to carry out in vitro culture to select optimal medium，also the best sterilization method and time were selected.The results indicated that 0.1% HgCl2 for 7 min was the best way.Its effects were better than 10% NaClO.The appropriate cultured medium for regeneration was MS+BA 2.0 mg/L+NAA 0.1 mg/L+KT 2.0 mg/L and the best root-induced cultured medium was 1/2MS+IBA 0.6 mg/L.All of the complete plants with roots were grown in the soil were survived.
参考文献/References:
[1]李瑜明,那宁馨,齐秀兰,等.红王子锦带的芽诱导及快速繁殖[J].中国林副特产,2002(3):44.
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备注/Memo
第一作者简介：李芳（1983-)，女,博士,研究方向为果树生理与分子生物学。E-mail：a2006055001@163.com。