[1]寇亚平,张施君,熊友华,等.春秋姜黄组培快繁技术研究[J].北方园艺,2012,36(07):142-144.
　KOU Ya-ping,ZHANG Shi-jun,XIONG You-hua,et al.Study on Tissue Culture and Rapid Propagation of Curcuma attenuta[J].Northern Horticulture,2012,36(07):142-144.
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春秋姜黄组培快繁技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年07期 页码: 142-144 栏目: 出版日期: 2012-07-16
Title:
Study on Tissue Culture and Rapid Propagation of Curcuma attenuta
文章编号:
1001-0009（2012）07-0142-03
作者:
寇亚平; 张施君; 熊友华; 刘念
仲恺农业工程学院 园林园艺学院，广东 广州 510225
Author(s):
KOU Ya-ping; ZHANG Shi-jun; XIONG You-hua; LIU Nian
College of Horticultural and Landscape Architecture，Zhongkai University of Agriculture and Engineering，Guangzhou，Guangdong 510225
关键词:
春秋姜黄; 腋芽; 组培快繁
Keywords:
Curcuma attenuta; axillary bud; tissue culture and rapid propagation
分类号:
S 682.2+9
文献标志码:
A
摘要:
以春秋姜黄根茎腋芽为外植体，采用添加6-BA、NAA、TDZ不同浓度和不同组合的MS基本培养基，进行不定芽诱导、丛生芽继代增殖和生根、壮苗培养等研究，探索其组织培养和离体快繁技术的适宜条件。结果表明：MS+6-BA 2.0 mg/L+NAA 0.5 mg/L的培养基腋芽诱导率高达86.7%，且生长速度快；MS+6-BA 2.0 mg/L+TDZ 0.1 mg/L 最有利于芽增殖，继代3次后，增殖系数达14.1；MS+6-BA 1.0 mg/L+NAA 0.5 mg/L培养基的壮苗生根效果最好。试管苗长至6 cm时移栽，成活率可达90%以上。
Abstract:
Taking rhizomes axillary buds of Curcuma attenuata as explants with adding 6-BA,NAA,TDZ different concentration and various combinations of MS basic medium,the optimal conditions of tissus culture and rapid propagation technology about shooting induction,multiple shoot subculture,proliferation,rooting,and seddling cultivation were studied.The results showed that the optimal medium for shooting induction was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L with an inducing rate of 86.7% and rapid growth.The proliferation efficiency of adventitious shoots increased 14.1-fold after subculture for three times on MS medium containing 2.0 mg/L 6-BA and 0.1 mg/L TDZ.Medium containing 1.0 mg/L 6-BA and 0.5 mg/L NAA served as the rooting medium.Over 90% of the plantlets survived when they grow more than 6 cm tall.

参考文献/References:

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备注/Memo

第一作者简介：寇亚平（1988-），女，在读硕士，研究方向为园林植物与观赏园艺及植物遗传育种。E-mail：kou_yaping@163.com。

基金项目：仲恺农业工程学院研究生创新基金资助项目（H1410011）。
收稿日期：2012-02-01

更新日期/Last Update: 2014-09-03